The Nanoblades: shuttles to operate the genome

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To precisely edit the genome, researchers can now use the CRISPR/Cas9 "genetic scissors", a very promising tool for gene therapy. The technological challenge today is to bring this tool to the genome of certain cells. To this end, the team of  Emiliano Ricci at the International Infectious Diseases Research Centre (CIRI) and at the Laboratoire de Biologie et Modélisation de la Cellule (LBMC) has developed “Nanoblades”, which are capsules that bring CRISPR/Cas9 to the target DNA, a work to which Aline Marnef in Gaelle Legube’s team at the LBCMCP contributed. This technology will facilitate the genetic modification of primary cells and open up opportunities for research on the editing of the human stem cell genome.

More information (in french) : CNRS press release

Reference

Genome editing in primary cells and in vivo using viral-derived “Nanoblades” loaded with Cas9/sgRNA ribonucleoproteins

Philippe E. Mangeot, Valérie Risson, Floriane Fusil, Aline Marnef, Emilie Laurent, Juliana Blin, Virginie Mournetas, Emmanuelle Massouridès, Thibault J. M. Sohier, Antoine Corbin, Fabien Aubé, Marie Teixeira, Christian Pinset, Laurent Schaeffer, Gaëlle Legube, François-Loïc Cosset, Els Verhoeyen, Théophile Ohlmann & Emiliano P. Ricci

Nature Communications

Researcher contact

Aline Marnef, chercheuse CNRS au Laboratoire de biologie cellulaire et moléculaire du contrôle de la prolifération (LBCMCP - CBI, CNRS/UT3 - Paul Sabatier)