Multi-tissue analysis of axis elongation in vertebrate embryo

Bertrand BENAZERAF

CR CNRS
05 61 55 67 39

Team members

  Technical staff
  • Guillaume ALLIO
  PhD
  • Michele ROMANOS

Presentation

 

Background

Understanding the formation of embryonic shapes is one of the fundamental themes in developmental biology and an important challenge in order to better understand developmental pathologies. Posterior axis elongation is a major morphogenetic event that produces the typical head-to-tail elongated body shape of vertebrate embryos. Axis elongation involves the three germ layers of the embryo: the ectoderm, the mesoderm, and the endoderm. However, while the mechanisms of tissue elongation have mostly been studied in separate tissue types, the principles allowing for the coordination of elongation between tissues and between germ layers remain largely unknown. 
 

Ongoing work- Emerging Team :

Using transgenic quail embryos that ubiquitously express a nuclear fluorescent protein and time-lapse imaging we recently analyzed and compared different tissue movements in the elongating embryo. This approach allowed us to demonstrate that embryonic elongation is defined by the coordination of distinct tissue-specific behaviors and extensive sliding between tissues. Further quantification of tissue tectonics showed tissue-specific patterns of rotations, contractions and expansions. By using a combination classical embryological techniques, live imaging, image analysis and mathematical modeling we are currently exploring the links between cell behaviors and tissue deformations. This multidiciplinary approach will allow us to decipher how cellular behaviors are coordinated in space and time to build the future organs in the embryo.  

 

Publications

  • Bénazéraf B..
    Dynamics and mechanisms of posterior axis elongation in the vertebrate embryo
    Cellular and Molecular Life Science
    2018 Sep review
  • Bertrand Bénazéraf*, Mathias Beaupeux, Martin Tchernookov, Allison Wallingford, Tasha Salisbury, Amelia Shirtz, Andrew Shirtz, David Huss, Olivier Pourquié*, Paul François*, Rusty Lansford*.
    Multiscale quantification of tissue behavior during amniote embryo axis elongation
    Development
    2017 Dec * co-coresponding authors
  • Huss D., Bénazéraf B., Wallingford A., Filla M., Yang J., Fraser S., Lansford R.
    A transgenic quail model that enables dynamic imaging of amniote embryogenesis.
    Development
    2015 Aug
  • Bénazéraf B, Pourquié O. .
    Formation and segmentation of the vertebrate body axis.
    Annual Review in Cell and Developmental Biology
    2013 Jun review
  • Bénazéraf B, Francois P, Baker RE, Denans N, Little CD, Pourquié O..
    A random cell motility gradient downstream of FGF controls elongation of an amniote embryo
    Nature
    2010 Jul

Funding

       

Université Paul Sabatier
118 Route de Narbonne

31062 TOULOUSE Cedex
France

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