Chromatin and Gene Expression

Kerstin Bystricky

Professeur, UPS
05 61 33 58 31

Team members

  • Anne-Claire Lavigne
  • Nicolas Tanguy Le Gac
  • Silvia Kocanova
  Technical staff
  • Isabelle Goiffon
  • Fatima Moutahir
  • Noémie Kempf
  • Thomas Germier
  • Ludmila Recoules
  Master Student
  • Aurore Fontaine
  • Flavien Raynal


We want to get a high resolution climpse at chromatin structure and chromosome behavior with respect to fundamental aspects of double strand break processing, transcription activation and viral infection. We develop methods to label DNA in fixed and living cells using fluorescent probes and develop non-invasive labeling techniques to visualize and quantify DNA. The technology transfer from yeast to human cells is an asset in the laboratory, and opens new perspectives in the field of chromatin dynamics. Applying mathematical modeling, we attempt to elucidate the functional relationship between the location of a gene, its inherent dynamics and its response to stimuli. We explore kinetics of long range chromatin interactions in order to obtain new structural insights on chromatin organization based on physical parameters. We analyse epigenetic mechanisms of gene expression in mammary tumor cells, investigating in particular the chromatin structure and its modifications at estrogen receptor target genes, and biophysical properties of chromosomes and their role in DNA double strand break repair in yeast. Our challenge is to experimentally act upon structure to probe impact on function.




Chromatin dynamics and regulation of gene expression

Cancer chromatin signatures – role of histone variants

Chromosome dynamics and DNA repair


  • Haitham Ahmed Shaban#$, Roman Barth$, Kerstin Bystricky#.
    Formation of correlated chromatin domains at nanoscale dynamic resolution during transcription
    Nucleic Acid Research
    2018 Apr in press; bioRxiv
  • Silvia Kocanova#, Isabelle Goiffon and Kerstin Bystricky#.
    3D FISH to analyse gene domain-specific chromatin re-modeling in human cancer cells
    2018 Mar doi:10.1016/j.ymeth.2018.02.013
  • Gomez-Lamarca MJ, Falo-Sanjuan J, Stojnic R, Rehman SA, Muresan L, Cerda-Moya G, Jones ML, Pillidge Z, Yuan Z, Valenti P, Bystricky K, Payre F, O’Holleran K, Kovall R, Bray S .
    Activation of the Notch signalling pathway in vivo elicits changes in CSL nuclear dynamics
    Developmental Cell
    2018 Feb 44 (1-16) 2018,
  • Thomas Germier, Silvia Kocanova, Nike Walther, Aurélien Bancaud, Hafida Sellou, Alessandro.Z.Politi, Jan Ellenberg, Franck Gallardo and Kerstin Bystricky.
    Real-time imaging of a single gene reveals transcription-initiated local confinement
    Biophysical Journal
    2017 Oct Volume 113, Issue 7, 3 October 2017, Pages 1383–1394
  • Aurélien Bancaud, Olivier Gadal, Kerstin Bystricky.
    Capturing chromosome structural properties from their spatial and temporal fluctuations
    Epigenetics and Systems Biology Chapter 12. , 2017
    2017 May doi:

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Université Paul Sabatier
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