Chromatin and Gene Expression
Professeur, UPS 05 61 33 58 31
Professor Technical staff
- Anne-Claire Lavigne
- Nicolas Tanguy Le Gac
- Isabelle Goiffon
- Fatima Moutahir
- Flavien Raynal
- Thomas Germier
- Ludmila Recoules
We want to get a high resolution climpse at chromatin structure and chromosome behavior with respect to fundamental aspects of double strand break processing, transcription activation and viral infection. We develop methods to label DNA in fixed and living cells using fluorescent probes and develop non-invasive labeling techniques to visualize and quantify DNA. The technology transfer from yeast to human cells is an asset in the laboratory, and opens new perspectives in the field of chromatin dynamics. Applying mathematical modeling, we attempt to elucidate the functional relationship between the location of a gene, its inherent dynamics and its response to stimuli. We explore kinetics of long range chromatin interactions in order to obtain new structural insights on chromatin organization based on physical parameters. We analyse epigenetic mechanisms of gene expression in mammary tumor cells, investigating in particular the chromatin structure and its modifications at estrogen receptor target genes, and biophysical properties of chromosomes and their role in DNA double strand break repair in yeast. Our challenge is to experimentally act upon structure to probe impact on function.
Chromatin dynamics and regulation of gene expression
Cancer chromatin signatures – role of histone variants
Chromosome dynamics and DNA repair
- Komatsu T., Quentin-Froignant C., Carlon-Andres I., Lagadec F., Rayne F., Ragues F., Kehlenbach R., Zhang W., Ehrhardt A., Bystricky K., Morin R., Lagarde JM., Gallardo F. and Wodrich H.# .
In vivo labelling of adenovirus DNA identifies chromatin anchoring and biphasic genome replication
Journal of Virology
2018 Sep DOI: 10.1128/JVI.00795-18
- Bernard Mariamé, Sandrine Kappler-Gratias, Martin Kappler, Stéphanie Balor, Franck Gallardo, and Kerstin Bystricky .
Real-time visualization and quantification of human Cytomegalovirus replication in living cells using the ANCHOR DNA labeling technology.
Journal of Virology
2018 Jun Accepted manuscript posted online 27 June 2018 , doi:10.1128/JVI.00571-18 Abstract Manuscript OPEN ACCESS ARTICLE
- Thomas Germier, Sylvain Audibert, Silvia Kocanova, David Lane and Kerstin Bystricky.
Real-time imaging of specific genomic loci in eukaryotic cells using the ANCHOR DNA labelling system
2018 May https://doi.org/10.1016/j.ymeth.2018.04.008
- Haitham Ahmed Shaban#$, Roman Barth$, Kerstin Bystricky#.
Formation of correlated chromatin domains at nanoscale dynamic resolution during transcription
Nucleic Acid Research
2018 Apr in press; https://doi.org/10.1101/230789 bioRxiv
- Silvia Kocanova#, Isabelle Goiffon and Kerstin Bystricky#.
3D FISH to analyse gene domain-specific chromatin re-modeling in human cancer cells
2018 Mar doi:10.1016/j.ymeth.2018.02.013
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SATT Toulouse Tech transfert
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