Presentation
SYNOPSIS
Béa Clouet-d’Orval, Marie Bouvier & Marta Kwapisz are co-supervizing the scientific projects of the team.
FOCUSES
The RNARCHAEA group, in close collaboration with local, national and international partners, aims at identifying and characterizing RNA metabolic pathways that are at the center of transcriptional and post-transcriptional gene expression regulation in archaea. In our interconnected, research projects we study novel enzymes and machineries that mediate RNA quality control, RNA stability and RNA processing pathways in Thermococcales study models.
Archaea, which constitute the third domain of life, are present in all ecosystems and represent more than 20% of the microbial biomass in ocean water and up to 5% of soil. They have various energy metabolism that makes them of crucial importance in Earth’s global bio-geochemical cycling. Archaea have been model systems for understanding life in extreme conditions, and insightful for the evolution of both molecular genetic processes and central metabolic pathways. Our study models, Thermococcus barophilus and Pyrococcus abyssi are a deep-sea hyperthermophile archaeons, which offer the unique possibility to address RNA, DNA and protein stability at extremely high temperatures (growth at temperatures above 85°C) and high pressure that challenge the frontiers of life.
Projets
Switch to survive - RNA metabolism during stationary transition in hyperthermophile archaea
RNA-degrading machines at the Ribosome in Archaea
Impact of the universally conserved ribonucleases from the β-CASP family on archaeal RNA metabolism
Publications
- Hadjeras L, Bouvier M, Canal I, Poljak L, Morin-Ogier Q, Froment C, Burlet-Schlitz O, Hamouche L, Girbal L, Cocaign-Bousquet M, Carpousis AJ..
Attachment of the RNA degradosome to the bacterial inner cytoplasmic membrane prevents wasteful degradation of rRNA in ribosome assembly intermediates
PLoS Biology
2023 Jan - Gaëlle Hogrel1,a, Sébastien Laurent2,3, Ziad Ibrahim1,b, Jacques Covès1, Eric Girard1, Frank Gabel1, Clarisse Etienne4, Marie-Claire Daugeron5, Béatrice Clouet-d’Orval4, Tamara Basta5, Didier Flament2,3, Bruno Franzetti1,*.
Characterization of a small tRNA-binding protein that interacts with the archaeal proteasome complex
Mol Microbiol . 2022 Jul;118(1-2):16-29. doi: 10.1111/mmi.14948. Epub 2022 Jun 15.
2022 Jul - Hajj M, Langendijk-Genevaux P, Batista M, Quentin Y, Laurent S, Capeyrou R, Abdel-Razzak Z, Flament D, Chamieh H, Fichant G, Clouet-d'Orval B, Bouvier M..
Phylogenetic Diversity of Lhr Proteins and Biochemical Activities of the Thermococcales aLhr2 DNA/RNA Helicase
Biomolecules
2021 Jun - D.K. Phung, C. Etienne, M. Batista, P. Langendijk-Genevaux, Y. Moalic, S. Laurent, S. Liuu, V. Morales, M. Jebbar, G. Fichant, M. Bouvier, D. Flament, B. Clouet-d’Orval.
RNA processing machineries in Archaea: the 5′-3′ exoribonuclease aRNase J of the β-CASP family is engaged specifically with the helicase ASH-Ski2 and the 3′-5′ exoribonucleolytic RNA exosome machinery
Nucleic Acids Research
2020 Feb - Manon Batista1, Petra Langendijk-Genevaux1, Mirna Hajj1,Duy Khanh Phung2, Laura Plassart3, Marie Bouvier1, Gwennaële Fichant1, and Béatrice Clouet-d’Orval1*.
The Archaeal Specific Helicase of the Ski2-like family, ASH-Ski2, is an authentic RNA helicase
2019 Dec en préparation
View all publications
Funding
Alumni
Mirna Hajj- former PhD
Manon Batista- former PhD
Duy Khanh Phung- former PhD
Bashir Saadek- former Post-doc
Laura Plassart- former master student
Dana Rinaldi