B. Clouet-d’Orval was appointed as a group leader of the “RNA biology in Archaea” project by the Scientific Committee of the CBI following the 2018 Emerging team Call
The team “RNA biology in Archaea”, in close collaboration with local, national and international partners aims at identifying and characterizing RNA metabolic pathways that are at the centre of gene expression regulation in Euryarchaea. We develop three interconnected, basic research projects to identify and characterize novel enzymes and machineries that mediate RNA quality control, RNA stability and RNA processing pathways using Thermococcales and Halophiles as study models:
-The first project focus is to decipher the functions of archaeal β-CASP RNases and associated RNA helicases and to identify RNA metabolic pathways at work in archaeal cells (ANR-CASPAR project, BCO coordinator).
-The second focus is to elucidate how the RNA and protein quality control are coupled and coordinated to fine-tune gene expression (ANR-PROTEIN Project, BCO partner).
-Finally, the third focus is to shed light on the molecular mechanisms that control the initial steps of RNA decay.
We believe that our work will provide knowledge that should prove to be milestones in the fields of RNA metabolism and molecular biology of Archaea, the third domain of life, and potentially clarify corresponding mechanisms in Eukarya and Bacteria. In addition, it should enlighten us on specific molecular processes that allow the archaeal cells to sustain an extremophile lifestyle.
Initial steps on mRNA decay in Thermococcales
Connection between the ribosome and the RNA modification/degradation machineries in Thermococcales
Impact of the universally conserved ribonucleases from the β-CASP family on archaeal RNA metabolism
- Hajj M., El-Hamaoui, S. Batista M.,Bouvier M., Abdel-Razzak, Z, Clouet-D’Orval, B*., and Chamieh, H.
Archaeal SF1 and SF2 helicases: Unwinding in the extreme
Helicases from all domains of life edited by Dr. Tutejah, Acadamic Press Elsevier,
2018 Dec Chap 1 pp 1-17 ISBN: 978-0-12-814685-9
- Hogrel G., Lu Y., Laurent S., Henry E., Phung DK., Bossé A., Pluchon P. F., Clouet-d’Orval B. and Flament D. .
Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus
Nucleic Acids Research,
2018 May vol 46 pp 5651-5663-doi: 10.1093/nar/gky322
- Clouet-d'Orval B*, Batista M, Bouvier M, Quentin Y, Fichant G, Marchfelder A, Maier LK.
Insights into RNA processing pathways and associated-RNA degrading enzymes in Archaea.
FEMS Microbiol Rev.
2018 Apr DOI:10.1093/femsre/fuy016
- Tomkuviene M, Ličytė J. , Olendraitė I; Clouet-d’Orval B, and Klimašauskas S .
Archaeal fibrillarin-Nop5 heterodimer 2'-O-methylates RNA independently of the C/D guide RNP particle
2017 Aug 23(9):1329-1337. doi: 10.1261/rna.059832
- Phung DK, Bouvier M., Clouet-d’Orval B*.
An overview of ribonuclease repertoire and RNA processing pathways in archaea
Nucleic Acids and Molecular Biology, Clouet-d’Orval B (ed.) – Springer International Publishing (A.W. Nicholson)
2017 May vol 32 Chap 4 pp 89-114, doi 10.1007/978-3-319-65795
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Duy Khanh Phung