We study how chromatin dynamics govern cell fate/proliferation through:
a) Nucleosome-positioning, which controls the accessibility of key genomic features such as gene promoters/bodies or replication origins.
b) Higher-order chromatin folding for the regulation of transcription/replication, which involves DNA looping to mediate long-range interactions between distinct/functional elements.
We investigate how specific chromatin modifiers (Mes-4/NSD, Hypb etc.), regulators (insulator and co-factors), and oncogenes (dref, …) impact transcription or replication through chromatin dynamics.
To this end, we employ biochemistry, molecular genetics, cellular biology combined with computational biology of high throughput sequencing data to tackle key aspects of chromatin organization.