Dynamic nuclear organization

Olivier GADAL


Chargé de Recherche, CNRS
05 61 33 59 39

Team members

  Researcher
  • Frederic BECKOUET
  • Christophe DEZ
  • Marta KWAPISZ
  Professor
  • Isabelle LEGER-SILVESTRE
  Technical staff
  • Christophe NORMAND
  PhD
  • Tommy DARRIERE
  • Lise DAUBAN
  Master Student
  • Emma LESAGE

Presentation

Our team studies how the genetic information is organized in the intranuclear space and how this spatial organization contributes to regulate gene expression.

Our model organism is the yeast Saccharomyces cerevisiae, and we focus on the early stages of ribosome biogenesis. The precursor of the major ribosomal RNAs produced by the RNA polymerase I initiates the assembly of the nucleolus, the largest nuclear compartment; the nucleolus is the nuclear body in which early pre-ribosomal particles assembly is achieved.

By combining quantitative tools of cell biology, biochemistry and global genetic screens, we identify the molecular components that contribute to intra-nuclear architecture. We try to identify organizational principles that dictate the spatial organization of eukaryotic genomes.

Projets

The role of the long non-coding RNAs in rDNA stability control

How is condensin recruited onto ribosomal DNA?

VISUALIZATION OF GENE TRANSCRIPTION AT SINGLE-MOLECULE RESOLUTION

Genetic tools to study nucleolus

Biophysical characterization of chromatin in yeast cell's nucleolus

Publications

  • Wang R, Normand C., Gadal O.
    High-Throughput Live-Cell Microscopy Analysis of Association Between Chromosome Domains and the Nucleolus in S. cerevisiae
    Methods Mol Biol - Nucleolus
    2016 Sep
  • Normand C., Berthaud M., Gadal O., Léger-Silvestre I.
    Correlative Light and Electron Microscopy of Nucleolar Transcription in Saccharomyces cerevisiae.
    Methods Mol Biol - Nucleolus
    2016 Sep
  • Belagal P, Normand C, Shukla A, Wang R, Léger-Silvestre I, Dez C, Bhargava P, Gadal O.
    Decoding the principles underlying the frequency of association with nucleoli for RNA polymerase III-transcribed genes in budding yeast
    Mol Biol Cell
    2016 Aug
  • Wang R, Mozziconacci J, Bancaud A, Gadal O.
    Principles of chromatin organization in yeast: relevance of polymer models to describe nuclear organization and dynamics
    Curr Opin Cell Biol
    2015 Jun
  • Hajjoul H, Mathon J, Ranchon H, Goiffon I, Mozziconacci J, Albert B, Carrivain P, Victor JM, Gadal O, Bystricky K, Bancaud A.
    High-throughput chromatin motion tracking in living yeast reveals the flexibility of the fiber throughout the genome
    Genome Res.
    2013 Nov

View all publications

Funding

 

IDEX Toulouse (CLEMGENE et NUDGENE), ANR-ANDY

Alumni

https://www-lbme.biotoul.fr/uk/equipes/grpgadal/alumni.html

Université Paul Sabatier
118 Route de Narbonne

31062 TOULOUSE Cedex
France

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