Our team studies how the genetic information is organized in the intranuclear space and how this spatial organization contributes to regulate gene expression.
Our model organism is the yeast Saccharomyces cerevisiae, and we focus on the early stages of ribosome biogenesis. The precursor of the major ribosomal RNAs produced by the RNA polymerase I initiates the assembly of the nucleolus, the largest nuclear compartment; the nucleolus is the nuclear body in which early pre-ribosomal particles assembly is achieved.
By combining quantitative tools of cell biology, biochemistry and global genetic screens, we identify the molecular components that contribute to intra-nuclear architecture. We try to identify organizational principles that dictate the spatial organization of eukaryotic genomes.
Wang R, Normand C., Gadal O. High-Throughput Live-Cell Microscopy Analysis of Association Between Chromosome Domains and the Nucleolus in S. cerevisiae Methods Mol Biol - Nucleolus 2016 Sep
Normand C., Berthaud M., Gadal O., Léger-Silvestre I. Correlative Light and Electron Microscopy of Nucleolar Transcription in Saccharomyces cerevisiae. Methods Mol Biol - Nucleolus 2016 Sep
Belagal P, Normand C, Shukla A, Wang R, Léger-Silvestre I, Dez C, Bhargava P, Gadal O. Decoding the principles underlying the frequency of association with nucleoli for RNA polymerase III-transcribed genes in budding yeast Mol Biol Cell 2016 Aug
Wang R, Mozziconacci J, Bancaud A, Gadal O. Principles of chromatin organization in yeast: relevance of polymer models to describe nuclear organization and dynamics Curr Opin Cell Biol 2015 Jun
Hajjoul H, Mathon J, Ranchon H, Goiffon I, Mozziconacci J, Albert B, Carrivain P, Victor JM, Gadal O, Bystricky K, Bancaud A. High-throughput chromatin motion tracking in living yeast reveals the flexibility of the fiber throughout the genome Genome Res. 2013 Nov