Spatio-temporal control of cell division

Sylvie TOURNIER

Yannick GACHET


DR2
05 61 55 81 13

DR2
05 61 55 81 13

Team members

  Technical staff
  • Céline REYES
  Postdoc
  • Simon CABELLO-AGUILAR
  PhD
  • Julien BERTHEZENE
  • Tong LI

Presentation

The accurate segregation of sister chromatids to daughter cells requires both the bipolar attachment of centromeres to the mitotic spindle and the correct positioning of the spindle with respect to the axis of cytokinesis. Cytokinesis always takes place perpendicularly to the axis of the spindle once chromosomes are segregated. These different events are coordinated in space and time in order to prevent the formation of aneuploid cells, a phenotype frequently observed in cancer and genetic diseases. The precise mechanisms leading to aneuploidy is unknown but is likely to be caused by either a defect in chromosome segregation, a defect in spindle positioning, a defect in cytokinesis or a combination of these defects. Although it is clearly established that faithful kinetochore/microtubule attachment is essential to prevent aneuploidy, several questions remain, such as:

What are the molecular mechanisms, which correct chromosome mis-attachment and how mis-attachment correction is coordinated with cytokinesis?

Using a multidisciplinary approach including genetic, video-microscopy and mathematical modeling techniques the aim of our lab is to identify the key players controlling chromosome segregation. We believe that our study performed in fission yeast will unveil new, basic and conserved mechanisms responsible for aneuploidy.

Projets

Modeling chromosome segregation and cell division

Identification of the mechanisms controlling aneuploidy

Publications

  • Cabello S, Gachet Y, Tournier S..
    Cutting edge science: Laser surgery illuminates viscoelasticity of merotelic kinetochores
    J Cell Biol. Mar 28;212(7):747-9.
    2016 Mar
  • Gachet Y, Reyes C, Tournier S..
    Aurora B kinase controls the separation of centromeric and telomeric heterochromatin
    Mol Cell Oncol.
    2015 Nov
  • Mary H, Fouchard J, Gay G, Reyes C, Gauthier T, Gruget C, Pécréaux J, Tournier S , Gachet Y .
    Fission yeast kinesin-8 controls chromosome congression independently of oscillations.
    J.CellSci. Oct 15;128(20):3720-30
    2015 Oct
  • 2015 Jan Le Bourg E, Gauthier T, Colinet H. Feeding on frozen live yeast has some deleterious effects in Drosophila melanogaster. Exp Gerontol. 2015; 7;69:202-210.
  • 2015 Jan Audry J, Maestroni L, Delagoutte E, Gauthier T, Nakamura TM, Gachet Y, Saintomé C, Géli V, Coulon S. RPA prevents G-rich structure formation at lagging-strand telomeres to allow maintenance of chromosome ends. EMBO J. 2015 14;34(14):1942-1958.

View all publications

Funding

         

Université Paul Sabatier
118 Route de Narbonne

31062 TOULOUSE Cedex
France

Annuaire général