Félicitation à Jean-Yves Bouet du LMGM-CBI Toulouse, lauréat de l’appel à Projet 80|Prime pour son projet NUMACOILED !
Cet appel à projet vise à soutenir et renforcer l’interdisciplinarité entre instituts du CNRS.
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Understanding the nucleation mechanism for the auto-assembly of a large functional nucleoprotein complex on supercoiled DNA.
1Coordinator: Jean-Yves BOUET, DR2-CNRS, INSB (Section CoNRS21)
2Partner: Jean-Charles Walter, CRCN-CNRS, INP (Section CoNRS02)
1 Laboratoire de Microbiologie et Génétique Moléculaires (LMGM), CBI, CNRS, Université de Toulouse, UPS, Toulouse, France
2 Laboratoire Charles Coulomb (L2C), CNRS-Université Montpellier, Montpellier, France
We are interested in the faithful inheritance of bacterial chromosomes and low-copy number plasmids by the major system driving active DNA segregation, namely the ParABS system. This project will focus on the auto-assembly of the large nucleoprotein complex that nucleates from the centromere-like site, parS. This so-called partition complexes result from the initial binding of a ParB dimer to parS that subsequently spatially confines hundreds of other ParB dimers inside the nucleoid. From our in vivo and in vitro data and physical modeling, we have recently proposed a new model, named ‘Nucleation & caging’ (Sanchez et al., 2015; Debaugny et al., 2018) to describe this auto-assembly mechanism. To get further insights on this assembly mechanism, we now aim (i) at understanding how only the ParB dimer bound to parS becomes competent to recruit all other ParB, and (ii) at implementing our modeling with a key element of the DNA organization in vivo, the supercoiling. The experimental part will mainly involve genetic, microscopy and ChIP-sequencing approaches, and the theoretical part will involve numerical simulations of ring polymer with supercoiling at the base pair resolutions together with analytical calculations.
Contact: Jean-Yves Bouet