Biologie de l'ARN chez les Archées

Béatrice Clouet-D´Orval

Chargé de Recherche, CNRS
05 61 33 58 75

Membres de l'équipe

  Chercheur  Soutien technique
  • Isabelle Canal
  Etudiant en Thèse
  • Manon Batista
  • Clarisse Etienne
  • Mirna Hajj

Présentation

SYNOPSIS

B. Clouet-d’Orval was appointed as a group leader of the “RNA biology in Archaea” project by the Scientific Committee of the CBI following the 2018 Emerging team Call

FOCUSES

The team “RNA biology in Archaea”, in close collaboration with local, national and international partners aims at identifying and characterizing RNA metabolic pathways that are at the centre of gene expression regulation in Euryarchaea. We develop three interconnected, basic research projects to identify and characterize novel enzymes and machineries that mediate RNA quality control, RNA stability and RNA processing pathways using Thermococcales and Halophiles as study models:

-The first project focus is to decipher the functions of archaeal β-CASP RNases and associated RNA helicases and to identify RNA metabolic pathways at work in archaeal cells (ANR-CASPAR project, BCO coordinator).

-The second focus is to elucidate how the RNA and protein quality control are coupled and coordinated to fine-tune gene expression (ANR-PROTEIN Project, BCO partner).

-Finally, the third focus is to shed light on the molecular mechanisms that control the initial steps of RNA decay.

We believe that our work will provide knowledge that should prove to be milestones in the fields of RNA metabolism and molecular biology of Archaea, the third domain of life, and potentially clarify corresponding mechanisms in Eukarya and Bacteria. In addition, it should enlighten us on specific molecular processes that allow the archaeal cells to sustain an extremophile lifestyle.

Projets

Initial steps on mRNA decay in Thermococcales

Connection between the ribosome and the RNA modification/degradation machineries in Thermococcales

Impact of the universally conserved ribonucleases from the β-CASP family on archaeal RNA metabolism

Publications

  • Hajj M., El-Hamaoui, S. Batista M.,Bouvier M., Abdel-Razzak, Z, Clouet-D’Orval, B*., and Chamieh, H.
    Archaeal SF1 and SF2 helicases: Unwinding in the extreme
    Helicases from all domains of life edited by Dr. Tutejah, Acadamic Press Elsevier,
    2018 Dec Chap 1 pp 1-17 ISBN: 978-0-12-814685-9
  • Hogrel G., Lu Y., Laurent S., Henry E., Phung DK., Bossé A., Pluchon P. F., Clouet-d’Orval B. and Flament D. .
    Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus
    Nucleic Acids Research,
    2018 May vol 46 pp 5651-5663-doi: 10.1093/nar/gky322
  • Clouet-d'Orval B*, Batista M, Bouvier M, Quentin Y, Fichant G, Marchfelder A, Maier LK.
    Insights into RNA processing pathways and associated-RNA degrading enzymes in Archaea.
    FEMS Microbiol Rev.
    2018 Apr DOI:10.1093/femsre/fuy016
  • Tomkuviene M, Ličytė J. , Olendraitė I; Clouet-d’Orval B, and Klimašauskas S .
    Archaeal fibrillarin-Nop5 heterodimer 2'-O-methylates RNA independently of the C/D guide RNP particle
    RNA
    2017 Aug 23(9):1329-1337. doi: 10.1261/rna.059832
  • Phung DK, Bouvier M., Clouet-d’Orval B*.
    An overview of ribonuclease repertoire and RNA processing pathways in archaea
    Nucleic Acids and Molecular Biology, Clouet-d’Orval B (ed.) – Springer International Publishing (A.W. Nicholson)
    2017 May vol 32 Chap 4 pp 89-114, doi 10.1007/978-3-319-65795

Voir toutes les publications

Financements

       

Anciens membres

Duy Khanh Phung

Dana Rinaldi

Bashir Saadeh

Kounthéa Phok

Université Paul Sabatier
118 Route de Narbonne

31062 TOULOUSE Cedex
France

Annuaire général