Offres de Post Doc

Single Molecule Analysis of a Homologous DNA Recombination Nanomachine.

Type de contrat : Post-doc-1 year -IDEX funded
Date de début : 2017-01-01
Equipe : Polard
Expérience demandée : Applicants should have a PhD in a relevant area of molecular, cellular life sciences or biophysics. Expertise in biochemistry, biophysics, single molecule approaches, or microfluidics approaches are highly advantageous but not mandatory. Applicants will also benefit from knowledge or research experience in the field of DNA repair or recombination.
Rémuneration :

 

A new postdoctoral position is available in the laboratory of Dr Patrice Polard at the LMGM (Pneumococcal team-Laboratory of Molecular Genetics & Microbiology) in Toulouse, South of France. This position is offered in the context of the project IDEX Emergence, SMART: Single-molecule analysis of homologous recombination during genetic transformation in the human pathogen Streptococcus pneumoniae.

 

Our research team aims to understand the mechanisms driving natural genetic transformation in the human pathogen Streptococcus pneumoniae at a molecular level. Transformation involves internalization of exogenous DNA as single strands, and promotes the acquisition of new genetic traits, mediating spread of antibiotic resistance or vaccine escape. It leads to the integration of exogenous DNA into the bacterial chromosome by homologous recombination (HR), a key and common step of multiple processes of genome biology in living organisms. Bacterial HR is catalyzed by the polymeric enzyme RecA, widely conserved in the three domains of life (named Rad51 in eukaryotes, RadA in Archea). In the context of transformation, RecA is regulated by a distinct set of proteins with which it defines a dedicated HR machine acting on transforming DNA. We are developing a single molecule approach combining TIRF (Total Internal Reflection Fluorescence) Microscopy and microfluidics to study the dynamics of this RecA-DNA machine of pneumococcal transformation in real time at the single molecule level. The successful post-doctoral applicant will participate in this project devoted for the characterization of protein activities on DNA.

Applicants should have a PhD in a relevant area of molecular, cellular life sciences or biophysics. Expertise in biochemistry, biophysics, single molecule approaches, or microfluidics approaches are highly advantageous but not mandatory. Applicants will also benefit from knowledge or research experience in the field of DNA repair or recombination.

The research group is located in LMGM, and benefits from the scientific environment of the new CBI-Toulouse (http://cbi-toulouse.fr/)

 

This project will be supervised by Maud Hertzog, a senior researcher in the team:

To apply, please reply to maud.hertzog@ibcg.biotoul.fr with the following information:

  • CV with list of publications
  • Names and contact details of two scientists who can write letters of recommendation

The position is available starting first of January 2017 (mandatory) for one year of salary funded by IDEX. Applications will be considered on an ongoing basis until the position is filled. The successful applicant will be encouraged to apply to post-doctoral fellowships, or could benefit from potential lab funding.




Biological functions of imprinted small non-coding RNA genes

Type de contrat : FRM
Date de début : 2016-10-01
Equipe : Cavaille
Expérience demandée :
Rémuneration :

 

Description - A 3 year post-doctoral research position is available in the group of Jérôme CAVAILLE at the Laboratory of Eukaryotic Molecular Biology (LBME) in Toulouse. Our research focuses on the function of hundreds of small noncoding RNAs specific to placental mammals and belonging to the microRNA and C/D snoRNA families. These genes map to chromosomal regions regulated by genomic imprinting, an epigenetic phenomenon by which genes are mono-allelically expressed in a parent-of-origin specific manner. The aim of our ongoing research is to explore the impact of these imprinted RNA genes on the expression and function of mammalian genomes, including in pathological contexts such as the Prader-Willi syndrome. The successful candidate will use novel knockout mouse models and next-generation sequencing (NGS)-based methods to explore the molecular and physiological roles of imprinted small regulatory RNA genes.

For further information - Labialle and Cavaillé (2011) Bioessays; Labialle, Marty (2014) EMBO J; Marty et al (2016) Hum Mol Genet. 

Keywords - Genomic imprinting, Prader-Willi Syndrome (PWS), knockout mouse model, C/D small nucleolar RNAs (SNORDs), microRNAs, post-transcriptional regulation.

Scientific environment – Toulouse is a 120,000-student town comprising three universities and several major engineering schools, which makes it the second university town in France. LBME is part of The Center for Integrative Biology in Toulouse (CBI-Toulouse) that regroups five research departments that form a strong pole in post-genomic biology, with a total of about 400 researchers (http://cbi-toulouse.fr).

Applicant profile - We are seeking a highly-motivated candidate with strong experiences in RNA biology, notably in NGS-based methods in relation to post-transcriptional regulation. Experience in epigenetics and/or mouse genetics would ideally complement this profile.

Contact – Candidates should send a cover letter describing their research interests, a CV including a list of publications and the names/emails of two (or three) references to cavaille@ibcg.biotoul.fr. Informal enquiries (via email) are welcome.

Deadline for Application – August 2016




Function and innervation of a novel type of muscles connecting internal organs

Type de contrat :
Date de début : 2016-09-01
Equipe : Vincent/Crozatier
Expérience demandée : Genetics Imaging and image analysis. Expertise in neural development would be a plus.
Rémuneration :

Centre for Developmental Biology, CBI Toulouse, France; http://cbi-toulouse.fr/eng/

Team: Gene regulatory networks in Drosophila haematopoiesis and myogenesis. http://cbi-toulouse.fr/eng/equipe-vincent-crozatier

Our team studies muscle development, using Drosophila as a model. We recently discovered a novel type of “deformable” muscles connecting visceral organs to the skeleton, that we named Thoracic Alary-Related Muscles (TARMs) (Boukhatmi et al., Development 2014). We hypothesize that TARMs play an architectural role in maintaining proper topological relations between the visceral, respiratory and circulatory systems during larval locomotion, reminiscent of the diaphragm in mammals.

The aim of the proposed project is to elucidate TARMs function and innervation, using genetic/transgenic strategies. It includes high resolution live-imaging of TARMs and their connections to internal organs and setting up functional assays in larvae (e.g. swallowing and navigational behavior) to analyze the consequences of TARMs ablation.

Highly motivated candidates should send their CV, a brief description of their research interests and contact information for 3 references to:

Alain VINCENT (alain.vincent@univ-tlse3.fr)




Evolutional connections in bacteria and mitochondria

Type de contrat : ATIP-funded fellowship, two initial years extendable to three years
Date de début : 2016-05-01
Equipe : Ieva
Expérience demandée : Genetics, Biochemistry, Cell Biology
Rémuneration : Experience-based

Our team is interested in bacterial stress survival and host interaction. We focus on protein sorting in pathogenic and non-pathogenic Gram negative bacteria, and in evolutionarily related organelles of eukaryotic cells called mitochondria. In one project we aim to unveil the maintenance programs that ensure correct functioning of the bacterial outer membrane. This compartment forms an essential permeability barrier and functions as a platform for the secretion of virulence factors targeted to the host cell. The candidate will investigate how environmental stresses trigger protein quality control in the bacterial outer membrane and how damaged and misfunctioning proteins are degraded. We believe this work will contribute to the research on novel antibiotics and vaccines against noxious bacteria.

The selected candidate will use complementary methodologies, including genetics, protein biochemistry (blue native PAGE, site-directed photo-crosslinking), and microscopy (epifluorescence, FRAP). Model organisms include Escherichia coli and Neisseria meningitidis. Candidates must have a PhD in a relevant research area and a strong expertise in microbial genetics, molecular/cellular biology or biochemistry.

A competitive salary (experience-based) will be offered. The position is funded by the joint CNRS/Inserm ATIP-Avenir program for two years, and it may be renewed for one additional year. The LMGM/CBI offers a dynamic work environment that hosts leading experts in the fields of prokaryotic and eukaryotic cell biology and is part of a large interdisciplinary campus at the Université Paul Sabatier (including mass spectrometry, crystallography, NMR platforms and high-throughput small molecule screening facilities). Toulouse is a culturally vibrant town in the south of France, close to the Pyrenees, the Mediterranean and the Atlantic coasts.




Université Paul Sabatier
118 Route de Narbonne

31062 TOULOUSE Cedex
France

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