To understand how ribosomal subunits are assembled, an international team including researchers from the LBME used different approaches integrating genetics, functional analysis and cryo-electron microscopy.
Cryo-EM allowed them to determine the atomic structure of pre-ribosomal particles purified at late stages of their maturation. They were thus able to show how events occurring at distant sites on the particle can be interconnected and synchronized, to allow the synthesis of functional ribosomes.
This study is published in the journal Nature Communications.
Read more (in french) : CNRS INSB website
Conformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism.
Mitterer V, Shayan R, Ferreira-Cerca S, Murat G, Enne T, Rinaldi D, Weigl S, Omanic H, Gleizes PE, Kressler D, Plisson-Chastang C, Pertschy B.
Nat Commun. 2019 Jun 21;10(1):2754. doi: 10.1038/s41467-019-10678-z.