Transcriptional control of cell fate specification by Mediator complex subunits during development

Presentation

The conserved multiprotein Mediator (MED) complex links DNA-bound transcription factors to RNA polymerase II (PolII). Despite a general role of Mediator in regulating nearly all PolII-dependent genes, studies from our and other labs have shown that a half of the 30 MED subunits have specific functions. Consistently, many human diseases have been linked to MED dysfunctions. Whereas most studies have been done from cultured cells, our team takes advantage of the Drosophila genetic model to investigate the molecular bases underlying the functional specificity of dedicated MED subunits in the transcriptional control of cell fate specification in-vivo.

Projets

Publications

• Clément Immarigeon 1 , Sandra Bernat-Fabre 1 , Emmanuelle Guillou 1 , Alexis Verger 2 , Elodie Prince 3 , Mohamed A Benmedjahed 1 , Adeline Payet 1 , Marie Couralet 4 , Didier Monte 2 , Vincent Villeret 2 , Henri-Marc Bourbon 1 , Muriel Boube 5.
  Mediator complex subunit Med19 binds directly GATA transcription factors and is required with Med1 for GATA-driven gene regulation in vivo
  J Biol Chem .
  2020 Jul
• Xiao Li 1 , Mengmeng Liu, Xingjie Ren, Nicolas Loncle, Qun Wang, Rajitha-Udakara-Sampath Hemba-Waduge, Stephen H Yu, Muriel Boube, Henri-Marc G Bourbon, Jian-Quan Ni, Jun-Yuan Ji.
  The Mediator CDK8-Cyclin C complex modulates Dpp signaling in Drosophila by stimulating Mad-dependent transcription
  PLoS Genet
  2020 May
• Xiao Li, Mengmeng Liu, Xingjie Ren, Nicolas Loncle, Qun Wang, Rajitha-Udakara-Sampath Hemba-Waduge, Stephen H. Yu, Muriel Boube, Henri-Marc G. Bourbon, Jian-Quan Ni, Jun-Yuan Ji .
  The Mediator CDK8-Cyclin C complex modulates Dpp signaling in Drosophila by stimulating Mad-dependent transcription
  PLOS GENETICS
  2020 May
• Immarigeon C, Bernat-Fabre S, Augé B, Faucher C, Gobert V, Haenlin M, Waltzer L, Payet A, Cribbs DL, Bourbon* HM, Boube* M.
  Drosophila Mediator Subunit Med1 Is Required for GATA-Dependent Developmental Processes: Divergent Binding Interfaces for Conserved Coactivator Functions
  Mol Cell Biol.
  2019 Mar
• Lacomme M, Medevielle F, Bourbon HM, Thierion E, Kleinjan DJ, Roussat M, Pituello F, Bel-Vialar S..
  A long range distal enhancer controls temporal fine-tuning of PAX6 expression in neuronal precursors.
  Dev Biol.
  2018 Apr

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Funding