Pneumococcal competence and transformation
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- Nathalie Campo
- Maud Hertzog
- Calum Johnston
- David Lane
- Patrice Polard
- Mathieu Berge
- Marc Prudhomme
- Violette Morales
- Isabelle Mortier
- Anne-lise Soulet
- Elise Courtais
- David De lemos
- Mickael Maziero
- Emeline Vernhes
- Robin Anger
The pneumococcal competence and transformation team combines complementary approaches to study all aspects of competence and genetic transformation in the human pathogen Streptococcus pneumoniae (the pneumococcus). Pneumococcal competence is a transient genetic programme induced in response to stresses. Comeptence promotes transformation, a mechanism of horizontal gene transfer entirely encoded by the recipient cell. It involves capture of exogenous DNA, internalization in the form of single strands and integration into the recipient chromosome by homologous recombination, driven by the recombinase RecA. This process is widespread in the bacterial kingdom and promotes acquisition of new genetic traits. In the pneumococcus, transformation can mediate the spread of antibiotics and vaccine escape. Our team combines molecular genetics, cell biology, biochemistry and single molecule analyses to study the pneumococcal transformation process. The main projects of our team focus on:
- The functional dissection of competence regulation.
- The functional dissection of the mechanism of transformation.
- A study of how competence and transformation are integrated into the cell cycle.
Through these complementary projects, we aim to further our fundamental understanding of this widespread, clinically relevant process.
Chromosome dynamics during pneumococcal competence for transformation
Pneumococcal competence regulation
Dissecting the TRAnsformation Machinery of the Pneumococcus (TRAM)
Single molecule analysis of recombination during transformation (SMART)
- De Ste Croix M, Chen K, Vacca I, Manso A, Johnston C, Polard P, Kwun M, Bentley S, Croucher N, Bayliss C, Haigh R, Oggioni M..
Recombination of the Phase-Variable spnIII Locus Is Independent of All Known Pneumococcal Site-Specific Recombinases.
Journal of Bacteriology
- Ramírez-Guadiana F, Rodrigues C, Marquis K, Campo N, Barajas-Ornelas R, Brock K, Marks D, Kruse A, Rudner D..
Evidence that regulation of intramembrane proteolysis is mediated by substrate gating during sporulation in Bacillus subtilis.
- Johnston, C. Mortier-Barriere, I., Khemici, V. Polard, P..
Fine-tuning cellular levels of DprA ensures transformant fitness in the human pathogen Streptococcus pneumoniae.
- Weyder M, Prudhomme M, Bergé M, Polard P, Fichant G..
Dynamic Modeling of Streptococcus pneumoniae Competence Provides Regulatory Mechanistic Insights Into Its Tight Temporal Regulation
2018 Jul 9:1637. doi:10.3389/fmicb.2018.01637.
- Weyder, M. Prudhomme, M. Berge, M, Polard, P. Fichant, G..
Dynamic Modeling of Streptococcus pneumoniae Competence Provides Regulatory Mechanistic Insights Into Its Tight Temporal Regulation.
Frontiers in Microbiology
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