PLATFORM
Responsable plateforme : Balor Stéphanie
Presentation
The Multiscale Electron Imaging (METi) platform is a transmission electron microscopy facility within the Centre de Biologie Intégrative de Toulouse (CBI).
The METi takes part to the “Toulouse Réseau Imagerie” (TRI-IBiSA) cell imaging network of the Génopole Toulouse-Midi-Pyrénées and obtained the ISO 9001:2015 – NFX50-900 certification in January 2010.
This facility is open to both academic and industrial users. Our expertise is centered on biology, but projects in other fields (material science, chemistry, geology,..) have been successfully carried out.
Major assignments
– Providing state-of-the-art technologies for Transmission Electron Microscopy (TEM)
– Developing innovative approaches for TEM and image analysis
– Transferring expertise through comprehensive training & teaching
Offers & Expertise
– Multiscale image analysis from tissue to molecule
– 3D Ultrastructural analysis
– Comprehensive Image Analysis in-situ and in-vitro
Access
– Fee for service & collaboration contract
– Equipment access after training
– For academic institutions and private companies
Prices/Procedures
– In order to ask a service and price please e-mail to cbi.meti@univ-tlse3.fr
TEM FEG 200 kV equipped with a direct electron detection camera with post-column energy-filter Gatan K3-BioQuantum and phase plate
TEM 120 kV equipped with a CMOS camera Gatan Rio 9 (9M pixels)
TEM 200 kV equipped with a US1000 camera and a US4000 camera with post-column energy-filter Gatan GIF Quantum
Automatic plunge freezer to freeze monodisperse particles (macromolecules, viruses, bacteria) in a thin layer of vitreous ice on carbon coated electron microscopy grids.
High pressure freezer to freeze samples in vitreous ice. It improves the morphological quality of the specimen, allows snapshots biological of short-lived processes, and improves preservation of antigens, which are often sensitive to chemical fixation.
Automatic freeze substitution and low temperature embedding system.
Cryo-ultramicrotome to perform ultrathin section at room temperature or more specifically dedicated for cryosectionning for Tokuyasu or CEMOVIS technique.
Talos Arctica
TEM FEG 200 kV equipped with a direct electron detection camera with post-column energy-filter Gatan K3-BioQuantum and phase plate
Jeol JEM 1400
TEM 120 kV equipped with a CMOS camera Gatan Rio 9 (9M pixels)
Jeol JEM 2100
TEM 200 kV equipped with a US1000 camera and a US4000 camera with post-column energy-filter Gatan GIF Quantum
Leica EMGP
Automatic plunge freezer to freeze monodisperse particles (macromolecules, viruses, bacteria) in a thin layer of vitreous ice on carbon coated electron microscopy grids.
Leica EM ICE
High pressure freezer to freeze samples in vitreous ice. It improves the morphological quality of the specimen, allows snapshots biological of short-lived processes, and improves preservation of antigens, which are often sensitive to chemical fixation.
Leica AFS2/FSP
Automatic freeze substitution and low temperature embedding system.
Leica UC7/FC7
Cryo-ultramicrotome to perform ultrathin section at room temperature or more specifically dedicated for cryosectionning for Tokuyasu or CEMOVIS technique.
Project 1
The METi is especially involved in the implementation of new technologies designed to prepare and observe samples as close as possible to their native state:
– Cryo-electron microscopy / Single particle analysis
– Cryomethods : cryofixation, freeze substitution and cryosections
– (Cryo) Electron tomography
Project 2
Developing comprehensive multiscale imaging to bridge the gap between molecules, cells and tissues for in situ structural biology analysis.
The latest developments in sample preparation, correlative microscopy approaches and cryo-electron tomography allow to depict molecular machines within their cellular context at an atomic level, and tend towards this within tissues or organs.
In this perspective, the next years we will focus on developing 3D volume EM approaches (Array tomography, cryoFIB-SEM).
Project 3
Our cryo-electron microscopy facility offers state-of-the-art image analysis services to reveal structural and quantitative details of biological samples, both in vitro and in situ. We specialize in:
Single particle analysis: Finding relative orientations and combining images of purified molecules to reconstruct high-resolution 3D structures of these particles.
Subtomogram averaging: This technique, currently under development at our facility, is ideal for studying large complexes present in multiple copies within a cell section (ribosome, proteasome, viruses…). It enhances signal and resolution by combining multiple tomogram slices, and allows to determine near-atomic resolution structures of macromolecules of interest within their cellular environment.
Segmentation: Identification and isolation of regions of interest within 3D tomographic volumes, enabling detailed analysis of cellular components and molecular complexes.
3D volume quantification and analysis: Measure molecular abundance, distribution and interactions in different environments, providing comprehensive insight into sample dynamics.
Our state-of-the-art computational techniques enable precise characterisation of molecular architecture and dynamics, supporting research into biological mechanisms.
– Integrative in vivo analysis of the ethanolamine utilization bacterial microcompartment in Escherichia coli. Jallet D, Soldan V, Shayan R, Stella A, Ismail N, Zenati R, Cahoreau E, Burlet-Schiltz O, Balor S, Millard P, Heux S.mSystems. 2024 Aug 20;9(8):e0075024. doi: 10.1128/msystems.00750-24. Epub 2024 Jul 18.
– Design, synthesis, and characterization of protein origami based on self-assembly of a brick and staple artificial protein pair. Moreaud L, Viollet S, Urvoas A, Valerio-Lepiniec M, Mesneau A, Li de la Sierra-Gallay I, Miller J, Ouldali M, Marcelot C, Balor S, Soldan V, Meriadec C, Artzner F, Dujardin E, Minard P.Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2218428120. doi: 10.1073/pnas.2218428120. Epub 2023 Mar 9.
– Elasticity of podosome actin networks produces nanonewton protrusive forces. Jasnin M, Hervy J, Balor S, Bouissou A, Proag A, Voituriez R, Schneider J, Mangeat T, Maridonneau-Parini I, Baumeister W, Dmitrieff S, Poincloux R.Nat Commun. 2022 Jul 4;13(1):3842. doi: 10.1038/s41467-022-30652-6.
– Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy. Shayan R, Rinaldi D, Larburu N, Plassart L, Balor S, Bouyssié D, Lebaron S, Marcoux J, Gleizes PE, Plisson-Chastang C.Molecules. 2020 Mar 3;25(5):1125. doi: 10.3390/molecules25051125.
Affiliation
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