Team
GSI
Team manager: Fichant Gwennaele
Presentation
Biological systems result from a complex evolutionary history, as partners and/or relationships between them may have been gained, lost or replaced during evolution. Our main research interest is to use genomic data to clarify the history that shaped key cellular process (ancestral genes, horizontal gene transfers, gene gains/losses) but also to provide functional information for experimental work. We address these issues by conducting comparative genomic analyses with phylogenomics approaches.
For several years, through different collaborations with experimental biologists, we have focused our studies on the evolution of RNA degradation and processing machineries in bacteria and archaea by conducting many phylogenomics analyses on the different partners (ribonuclease, helicases, …). More recently, we have initiated a collaboration with Peter Redder’s group to understand RNase J-mediated RNA degradation in Firmicutes with S. aureus as a model organism, and to assess the contribution of secondary structures and 5′ epi-transcriptomic modifications of RNA substrates to the regulation of this process. In addition to phylogenomics approaches on the partners of the degradation machinery, this project focuses on RNA substrates and requires the development of original workflows and statistical analyses to integrate the specificity of the RNA-Seq data produced by P. Redder’s team.
– Xu X, Barriot R, Voisin B, Arrowsmith TJ, Usher B, Gutierrez C, Han X, Pagès C, Redder P, Blower TR, Neyrolles O, Genevaux P. Nucleotidyltransferase toxin MenT extends aminoacyl acceptor ends of serine tRNAs to control Mycobacterium tuberculosis growth Nat Commun, 2024 Nov 15(1):9596. doi: 10.1038/s41467-024-53931-w. PMID: 39505885
– Le Scornet A, Jousselin A, Baumas K, Kostova G, Durand S, Poljak L, Barriot R, Coutant E, Pigearias R, Tejero G, Lootvoet J, Péllisier C, Munoz G, Condon C, Redder P. Critical factors for precise and efficient RNA cleavage by RNase Y in Staphylococcus aureus PLoS Genetics, 2024 Aug 1;20(8):e1011349. doi: 10.1371/journal.pgen.1011349. PMID: 39088561
– Batista M*, Langendijk-Genevaux P*, Kwapisz M, Canal I, Phung DK, Plassart L, Capeyrou R, Moalic Y, Jebbar M, Flament D, Fichant G, Bouvier M, Clouet-d’Orval B . Batista M, Langendijk-Genevaux P, Kwapisz M, Canal I, Phung DK, Plassart L, Capeyrou R,Evolutionary and functional insights into the Ski2-like helicase family in Archaea: a comparison of Thermococcales ASH-Ski2 and Hel308 activities NAR Genom Bioinform, 2024 Mar 6(1):lqae026. doi: 10.1093/nargab/lqae026. PMID: 38500564
– Yiying Yang, Haoxiang Chen, Robin A. Corey, Violette Morales, Yves Quentin, Carine Froment, Anne Caumont-Sarcos, Cécile Albenne, Odile Burlet-Schiltz, David Ranava, Phillip J. Stansfeld, Julien Marcoux, Raffaele Ieva. LptM promotes oxidative maturation of the lipopolysaccharide translocon by substrate binding mimicry . Nature Communications, 2023 Oct
– Xu X, Usher B, Gutierrez C, Barriot R, Arrowsmith TJ, Han X, Redder P, Neyrolles O, Blower TR, Genevaux P.. MenT nucleotidyltransferase toxins extend tRNA acceptor stems and can be inhibited by asymmetrical antitoxin binding Nat Commun, 2023 Aug 14(1):4644. doi: 10.1038/s41467-023-40264-3. PMID: 37591829
