PLATFORM
Presentation
The Mouse Behavioral Core (MBC) – CBI provides a wide range of behavioral tasks in mice, allowing studies on genetic and pharmacological models of human disorders, as well as preclinical testing of novel therapeutic agents.
The Mouse Behavioral Core offers:
– specialized areas for experimentation and behavioral studies
– cutting-edge equipment, software, and methodologies for comprehensive assessment of mouse behavior
– expertise in behavioral phenotyping, with support for executing experimental projects
– improvement and development of new behavioral protocols to address user’s specific scientific questions
– training on all available behavioral equipment, along with assistance in data analysis and interpretation
The MBC facility features 12 rooms dedicated to behavioral studies, 15 workstations, and over 30 different devices designed to evaluate various behavioral phenotyping in mice, as:
– Learning and memory:
Fear conditioning / Holeboard / Morris water maze / Object recognition and object location / Conditionned place preference / Passive and active avoidance / Automated Y-maze
– Social and communication:
5 trials social test / Three-chamber / Ultravocalization
– Sensorimotor:
Activity wheels / Grip test / Rotarod / Rotometer / Treadmill / Tight rope / Pre-pulse inhibition
– Anxiety and “depression”:
Elevated-plus maze / Tail-suspension test / Open-field / Novelty suppressed feeding / Looming and sweeping test / Splash test
– Nociception:
Von Frey / Hot plate
– Water and food consumption / motivation:
Actimeter with lickmeter and pellet dispenser
projects
MBC supports numerous scientific projects at the academic level (CNRS and INSERM) and in the private sector. For instance, the MBC has 3 ongoing projects:
Academic project: INSPIRE (Toulouse)
Define an appropriate animal model of frailty/accelerated aging, by measuring behavioral and physiological changes occurring at various ages in a large mouse cohort with different level of activity and food diet.
Academic project: I2MC/INSERM (Toulouse)
Evaluate muscle strength, motor and locomotor activity, endurance, and cognitive functions in mice lacking ATPase inhibitory factor 1 (IF1)
Industrial project: Enterosys (Toulouse)
Evaluate anxiety, motor and locomotor activity, resignation, and anhedonia (sucrose preference test) following a chronic unpredictable stress (CUS) protocol in mice
– Andraini T, Moulédous L, Petsophonsakul P, Florian C, Gauzin S, Botella M, Arrázola MS, Nikolla K, Philip A, Leydier A, Marque M, Pelloquin-Arnauné L, Belenguer P, Rampon C, Miquel M-C. (2023) Mitochondrial OPA1 deficiency is associated to reversible defects in spatial memory related to adult neurogenesis in mice. eNeuro. https://doi.org/10.1523/ENEURO.0073.23.2023
– Viguier C, Bullich S, Botella M, Fasseu L, Alfonso A, Rekik K, Gauzin S, Guiard B, Davezac N. (2023). Impact of physical activity on brain oxidative metabolism and intrinsic capacities in young swiss mice fed a high fat diet, Neuropharmacology. https://doi.org/10.1016/j.neuropharm.2023.109730
– Kermorgant M, Ben Salem J, Iacovoni JS, Calise D, Dahan L, Guiard BP, Lopez S, Lairez O, Lasbories A, Nasr N, Pavy Le-Traon A, Beaudry F, Senard JM, Arvanitis DN (2021). Cardiac sensory afferents modulate susceptibility to anxio-depressive behaviour in a mouse model of chronic heart failure. Acta Physiol (Oxf). 231(4). https://doi.org/10.1111/apha.13601
– Santin Y, Lopez S, …(… )…, Guiard BP, Parini A. Towards a Large-Scale Assessment of the Relationship between Biological and Chronological Aging: The INSPIRE Mouse Cohort. J Frailty Aging. 2021;10(2). https://doi.org/10.14283/jfa.2020.43
Affiliation
