Team
GeDy
Team manager: Bouet Jean-Yves & Cornet François
Our team studies the functions involved in maintenance and adaptability of the different replicons composing bacterial genomes, chromosomes and plasmids. We focus on the molecular mechanisms resolving physical links between sister replicons and their partitioning into daughter cells. In particular, we aim at understanding how these mechanisms are adapted to various context depending on replicon types and bacterial species, and how this allows the remarkable adaptability of bacteria. Our recent projects explore the core functions of ParABS type partitioning systems, mechanisms for resolving inter-catenated or dimerized chromosomes and plasmids alongside their coupling to cell division, and the role of site-specific recombination in genome plasticity and the acquisition of multidrug resistance genes by plasmids.
We use different bacteria, mainly Escherichia coli and Acinetobacter baumannii, which are among the most worrying pathogens considering their strong ability for acquiring antibiotic resistance.
We employ a combination of approaches, both in-house — mainly molecular genetics biochemistry, cell biology and bioinformatics — and through collaborations, including biophysics, theoretical physics, modelling and ecology.
Team members
– Blanchais et al. (2024) Interplay between the Xer recombination system and the dissemination of antibioresistance in Acinetobacter baumannii. bioRxiv 2024.04.09.588662; doi: https://doi.org/10.1101/2024.04.09.588662.
– Fournes et al. (2024) The pathway to resolve dimeric forms distinguishes plasmids from megaplasmids in Enterobacteriaceae. bioRxiv 2024.04.05.588136; doi: https://doi.org/10.1101/2024.04.05.588136.
– Sekkouri Alaoui et al. (2024) In vivo Assembly of Bacterial Partition Condensates on Supercoiled and Linear DNA. Mol. Microbiol. doi: 10.1111/mmi.15297.
– Cornet et al. (2023) DNA segregation in enterobacteria. EcoSalPlus ESP-0038-2020.
– Walter et al. (2020) Physical modeling of a sliding clamp mechanism for the spreading of ParB at short genomic distance from bacterial centromere sites. iScience 23: 101861.
– Crozat et al (2020) Post-replicative pairing of sister ter regions in Escherichia coli involves multiple activities of MatP. Nat Commun. 30;11(1):3796. doi: 10.1038/s41467-020-17606-6.
– Bouet and Funnell (2019) Plasmid localization and partition in Enterobacteriaciae. EcoSalPlus 8(2) ESP-0003-2019
Affiliation
