Following a thesis in oncology on the impact of the microenvironment on the regulation of the proliferation of normal and leukaemic cells, I performed a post-doctoral research on the characterisation of the physiological function of DNA polymerase Theta, a DNA polymerase overexpressed in many cancers.
Since 2015, I have been a assistant professor at the University of Toulouse. I mainly teach in the Pharmacy department of the Faculty of Health in the field of genotoxicology/oncology.
I first carried out my research at INRAE-Toxalim laboratory, where I studied the impact of xenobiotics (bacterial genotoxins and pesticides) on genome stability, before joining Dr Trouche's team at CBI/MCD in 2020. My current research project is based on identifying the specific functions of the three H2A.Z isoforms in maintaining genome stability during the cell cycle, in particular during the DNA replication phase and during Mitosis, and thus understanding the impact of their deregulation in cancers.
To this end, we have constructed U2OS cell lines in the laboratory by genome editing, enabling (i) to monitor the localisation of endogenous H2A.Z isoforms in real time as they progress through the cell cycle and (ii) to induce their degradation in a rapid, inducible and reversible manner in specific phases of the cell cycle. This approach will make it possible to decipher the mechanisms of action of H2A.Z isoforms in the regulation of genome stability and to understand the consequences of H2A.Z overexpression or mutations observed in cancers.
