Chromatin and gene expression

Présentation

Do chromatin structure, chromosome motion and genome organization regulate nuclear processes? To address this question we investigate the functional relationship between the location of a gene, its inherent dynamics and its response to stimuli. We analyze epigenetic mechanisms of gene expression in mammary tumor cells, investigating in particular the role of histone variants and 3D folding of chromatin domains at estrogen receptor target genes. We develop techniques to visualize and image DNA in living cells, at the level of single loci and the whole genome, at high resolution, and methods to quantitatively analyze and model the data. Our challenge is experimentally acting upon structure to probe impact on function.

Projets

Publications

• Kocanova S., Raynal F., Goiffon I., Agkol Oksuz B., Baú D., Kamgoué A., Cantaloube S., Zhang Y., Lajoie B., Marti-Renom M., Job Dekker J. and Bystricky K..
  Enhancer-driven local 3D chromatin domain folding modulates transcription in human mammary tumor cells
  Life Science Alliance
  2023 Dec doi.org/10.1101/2023.10.17.562690, 2023
• Ludmila Recoules, Nicolas Tanguy Le Gac, Fatima Moutahir, Kerstin Bystricky *, Anne-Claire Lavigne *.
  Recruitment of the histone variant macroH2A1 to the pericentric region occurs upon chromatin relaxation and is responsible for major satellite transcriptional regulation
  Cells 2023, 12(17), 2175
  2023 Aug
• Ludmila Recoules, Alexandre Heurteau, Flavien Raynal, Nezih Karasu, Fatima Moutahir, Fabienne Bejjani, Isabelle Jariel-Encontre, Olivier Cuvier, Thomas Sexton, Anne-Claire Lavigne, Kerstin Bystricky.
  The histone variant macroH2A1.1 regulates RNA Polymerase II paused genes within defined chromatin interaction landscapes
  J Cell Sci
  2022 Apr doi: 10.1242/jcs.259456.
• Guilherme M Oliveira, Attila Oravecz, Dominique Kobi, Manon Maroquenne, Kerstin Bystricky, Tom Sexton, Nacho Molina .
  Precise measurements of chromatin diffusion dynamics by modeling using Gaussian processes
  Nat Communications
  2021 Oct 12, 6184 (2021).
• Anis Meschichi, Mathieu Ingouff, Claire Picart, Marie Mirouze, Sophie Desset, Franck Gallardo, Kerstin Bystricky, Nathalie Picault, Stefanie Rosa, Frederic Pontvianne .
  ANCHOR, a technical approach to monitor single-copy locus localization in planta
  Front. Plant Sci.
  2021 Jul 06 July 2021

Voir toutes les publications

Financements

Kerstin BYSTRICKY, 2019 membre senior

International 4D Nucleome action CA18127

Anciens membres

Maitre de Conférences:
Silvia KOCANOVA, MCU depuis 2010, changement d'équipe 2022

Postdoc:

Noémie KEMPF, postdoc 3/17-12/19
Hafida SELLOU, postdoc 4/17-4/18 now postdoc Lyon
Haitham SHABAN, postdoc 8/15-3/18 now postdoc EPFL, Switzerland
Franck GALLARDO, postdoc 06/10-12/13 now CEO NeoVirTech SA, Toulouse
Mathieu DALVAI, postdoc 10/08-12/12 now senior scientist to the Director, Quebec, Canada

PhD students:

Ludmila RECOULES, PhD 07/2021 (PhD student 10/17-06/21)
Sylvain AUDIBERT, PhD 11/2017 (PhD student 10/13-11/17, ATER 16-17, AI 01-03/18) now postdoc Bristol, UK
Barbara LAMBOUR, PhD 04/2016 (PhD student 02/14-04/16)
Hicham SAAD, PhD 11/2013 now at NIKON Canada
Luca BELLUCCI, PhD 03/2013 now teacher college Haute Garonne
Imen LASSADI, PhD 08/2012 now senior scientist Cambridge, UK
Mahta MAZAHERI, PhD 05/2009 now MD/PhD breast cancer institute, Iran
Laurence FLEURY, PhD 04/2008 now IE Région Albi

Ingénieurs and technical personnel:

Isabelle GOIFFON, AI/IE CNRS 10/17-08/20
Flavien RAYNAL, AI bioinfo CDD 10/18-12/19
Perrine UHART, AI CDD 05/18-08/19
Stéphanie CAZE-SUBRA, IE CDD 09/16-5/17 now IE
Sandrine KAPPLER-GRATIAS, IE CDD 06/14-3/17 now at NeoVirTech SA
Laurence FLEURY, IE CDD 06/12-04/13 now IE Région Albi

Master, Licence and BTS students: