Do chromatin structure, chromosome motion and genome organization regulate nuclear processes? To address this question we investigate the functional relationship between the location of a gene, its inherent dynamics and its response to stimuli. We analyze epigenetic mechanisms of gene expression in mammary tumor cells, investigating in particular the role of histone variants and 3D folding of chromatin domains at estrogen receptor target genes. We develop techniques to visualize and image DNA in living cells, at the level of single loci and the whole genome, at high resolution, and methods to quantitatively analyze and model the data. Our challenge is experimentally acting upon structure to probe impact on function.
Chromatin dynamics and regulation of gene expression
Cancer chromatin signatures – role of histone variants
Chromosome dynamics and DNA repair
- Barth R., Bystricky K. and Shaban HA .
Coupling chromatin structure and dynamics by live super-resolution imaging
2020 Jul in press; bioRxiv doi.org/10.1101/777482
- Kempf N., Moutahir F., Goiffon I., Cantaloube S., Bystricky K. and Lavigne A.-C. .
Analysis of Cellular EMT States Using Molecular Biology and High Resolution FISH Labeling
Methods in Molecular Biology
2020 May chapter; Vol. 2179, Kyra Campbell and Eric Theveneau (Eds): The Epithelial-to Mesenchymal Transition
- Audibert S.*, Tanguy-le-Gac N.*, Rech J., Bouet J.Y., Bystricky K. and Lane D. .
Role of centromere sites in activation of ParB proteins for partition complex assembly
PLoS ONE 15(5):e0226472
2020 May 10.1371/journal.pone.0226472, 2020
- Shaban HA, Barth R, Recoules L, Bystricky K. .
Hi-D: nanoscale mapping of nuclear dynamics in single living cells.
2020 Apr 20;21(1):95. doi: 10.1186/s13059-020-02002-6.
- Hurtado-Bagès S., Posavec Marjanovic M., Valero V., Malinverni R., Corujo D., Lavigne A.-C., Bystricky K. and Buschbeck M..
The Histone Variant MacroH2A1 Regulates Key Genes for Myogenic Cell Fusion in a Splice-Isoform Dependent Manner
2020 Apr 9(5), 1109; https://doi.org/10.3390/cells9051109
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Kerstin BYSTRICKY, 2019 membre senior
International 4D Nucleome action CA18127
Hafida SELLOU, postdoc 4/17-4/18
Haitham SHABAN, postdoc 8/15-7/18
Franck GALLARDO, postdoc 06/10-12/13
Mathieu DALVAI, postdoc 10/08-12/12
Thomas GERMIER, PhD 11/2018 (PhD student 10/15-11/18)
Sylvain AUDIBERT, PhD 11/2017 (PhD student 10/13-11/17, ATER 16-17, AI 01-03/18)
Barbara LAMBOUR, PhD 04/2016 (PhD student 02/14-04/16)
Hicham SAAD, PhD 11/2013
Luca BELLUCCI, PhD 03/2013
Imen LASSADI, PhD 08/2012
Mahta MAZAHERI, PhD 05/2009
Laurence FLEURY, PhD 04/2008
Ingénieurs and technical personnel:
Stéphanie CAZE-SUBRA, IE 09/16-5/17 (now CDI Evotec Toulouse)
Sandrine KAPPLER-GRATIAS, IE 06/14-3/17 (now CDI NeoVirTech Toulouse)
Laurence FLEURY, IE 06/12-04/13 (now IR CNRS Albi territorial office)