Ribosome Biogenesis in Eukaryotes
DR CNRS 05 61 33 59 53
CR CNRS 05 61 33 59 55
Associate professor Technical staff
- Anthony Henras
- Yves Henry
- Odile Humbert
- Benjamin Albert
- Carine Dominique
- Jessie Bourdeaux
- Emilie Cerezo
- Mariam Jaafar
- Ali Khreiss
Ribosome production in eukaryotes is initiated by the transcription by RNA polymerase I of a pre-ribosomal RNA (pre-rRNA), precursor to the 18S, 5.8S and 25S/28S ribosomal RNAs (rRNAs). This pre-rRNA associates co-transcriptionally with ribosomal proteins, small nucleolar ribonucleoprotein particles (snoRNPs) and non-ribosomal proteins to yield a nascent pre-ribosomal particle from which the early pre-40S particle containing the 18S rRNA sequence is split mostly co-transcriptionally. The early pre-60S particle assembled onto the 5.8S and 25S sequences is released subsequently following transcription termination. These particles then follow independent maturation pathways, first in the nucleus and finally in the cytoplasm. Within this series of pre-ribosomal particles, the pre-rRNAs are chemically modified and progressively processed by endo- and exoribonucleases to release the mature rRNAs. Hundreds of snoRNPs and more than 200 non-ribosomal proteins are involved in ribosome synthesis in eukaryotes. The molecular functions of most of these co-factors, several of which are enzymes, remain elusive. Our aims are to understand the precise molecular functions and modes of regulation of several key non-ribosomal factors involved in the co- and/or post-transcriptional steps of ribosome synthesis, in particular energy-consuming enzymes.
Function of the atypical RIO kinases in late stages of the maturation of pre-40S particles in yeast and human cells
Function of the Dbp6p/Npa1p/Npa2p/Nop8p/Rsa3p complex in the maturation of early pre-60S particles in yeast
Regulation of the Prp43p helicase by its G-patch domain-containing co-factors Gno1p/PINX1 and Pfa1p in yeast and human
Function of Rpf2p and Rrs1p in the coordination between synthesis and maturation of the pre-ribosomal RNA in yeast and human
- Joret C, Capeyrou R, Belhabich-Baumas K, Plisson-Chastang C, Ghandour R, Humbert O, Fribourg S, Leulliot N, Lebaron S, Henras AK, Henry Y.
The Npa1p complex chaperones the assembly of the earliest eukaryotic large ribosomal subunit precursor
PLoS Genet. 14:e1007597
2018 Aug OPEN ACCESS. doi: 10.1371/journal.pgen.1007597
- Belhabich-Baumas K, Joret C, Jády BE, Plisson-Chastang C, Shayan R, Klopp C, Henras AK, Henry Y, Mougin A.
The Rio1p ATPase hinders premature entry into translation of late pre-40S pre-ribosomal particles
Nucleic Acids Res, 45, 10824-10836
2017 Oct OPEN ACCESS, doi: 10.1093/nar/gkx734
- Montellese C, Montel-Lehry N, Henras AK, Kutay U, Gleizes PE, O'Donohue MF.
Poly(A)-specific ribonuclease is a nuclear ribosome biogenesis factor involved in human 18S rRNA maturation
Nucleic Acids Res, 45, 6822-6836
2017 Apr OPEN ACCESS doi: 10.1093/nar/gkx253
- Henras AK, Plisson-Chastang C, Humbert O, Romeo Y, Henry Y.
Synthesis, Function, and Heterogeneity of snoRNA-Guided Posttranscriptional Nucleoside Modifications in Eukaryotic Ribosomal RNAs
Enzymes, 41, 169-213
2017 Apr OPEN ACCESS, doi: 10.1016/bs.enz.2017.03.007
- Robert-Paganin J, Halladjian M, Blaud M, Lebaron S, Delbos L, Chardon F, Capeyrou R, Humbert O, Henry Y, Henras AK, Réty S, Leulliot N..
Functional link between DEAH/RHA helicase Prp43 activation and ATP base binding.
Nucleic Acids Research, 45(3):1539-1552.
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