Ribosome Biogenesis in Eukaryotes

Presentation

Ribosome production in eukaryotes is initiated by the transcription by RNA polymerase I of a pre-ribosomal RNA (pre-rRNA), precursor to the 18S, 5.8S and 25S/28S ribosomal RNAs (rRNAs). This pre-rRNA associates co-transcriptionally with ribosomal proteins, small nucleolar ribonucleoprotein particles (snoRNPs) and non-ribosomal proteins to yield a nascent pre-ribosomal particle from which the early pre-40S particle containing the 18S rRNA sequence is split mostly co-transcriptionally. The early pre-60S particle assembled onto the 5.8S and 25S sequences is released subsequently following transcription termination. These particles then follow independent maturation pathways, first in the nucleus and finally in the cytoplasm. Within this series of pre-ribosomal particles, the pre-rRNAs are chemically modified and progressively processed by endo- and exoribonucleases to release the mature rRNAs. Hundreds of snoRNPs and more than 200 non-ribosomal proteins are involved in ribosome synthesis in eukaryotes. The molecular functions of most of these co-factors, several of which are enzymes, remain elusive. Our aims are to understand the precise molecular functions and modes of regulation of several key non-ribosomal factors involved in the co- and/or post-transcriptional steps of ribosome synthesis, in particular energy-consuming enzymes.

Projets

Publications

• Cerezo EL, Houles T, Lié O, Sarthou MK, Audoynaud C, Lavoie G, Halladjian M, Cantaloube S, Froment C, Burlet-Schiltz O, Henry Y, Roux PP, Henras AK, Romeo Y..
  RIOK2 phosphorylation by RSK promotes synthesis of the human small ribosomal subunit.
  PLoS Genetics
  2021 Jun
• Mouffok S, Capeyrou R, Belhabich-Baumas K, Joret C, Henras AK, Humbert O, Henry Y..
  The G-patch activators Pfa1 and PINX1 exhibit different modes of interaction with the Prp43 RNA helicase.
  RNA Biology
  2021 Apr
• Hantelys F, Godet AC, David F, Tatin F, Renaud-Gabardos E, Pujol F, Diallo LH, Ader I, Ligat L, Henras AK, Sato Y, Parini A, Lacazette E, Garmy-Susini B, Prats AC..
  Vasohibin1, a new mouse cardiomyocyte IRES trans-acting factor that regulates translation in early hypoxia.
  Elife
  2019 Dec
• Dauban L, Cerezo E, Henras A, Gadal O..
  Meeting report from the first European OddPols meeting: Toulouse 2018.
  Gene
  2019 Jun
• Albert B, Kos-Braun IC, Henras AK, Dez C, Rueda MP, Zhang X, Gadal O, Kos M, Shore D..
  A ribosome assembly stress response regulates transcription to maintain proteome homeostasis.
  Elife
  2019 May

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Funding