Gut Development and Homeostasis

Presentation

Our research is aimed towards understanding the regulatory principles of epithelial renewal in the mammalian gut — how this astonishingly dynamic tissue is initially established during development, and how it is maintained in adult tissue homeostasis, using the mouse gut as a model.

We focus on the regulatory role of mechanical forces in establishment and maintenance of epithelial turnover and integrity, integrating cell- and tissue-level mechanics with the physiological context of the mammalian gut.

Our experimental approach combines live tissue imaging (in vivo and ex vivo) with genetic, pharmacological and force perturbations and theoretical modelling.

We welcome applications from prospective postdoc and PhD candidates, with a strong background in cell & developmental biology. 
Postdoc candidates should send their application including CV, and statement of motivation and research interests to denis.krndija[at]univ-tlse3.fr

Projets

Establishment of epithelial renewal during gut development

Maintenance of gut epithelial renewal and integrity

Mechanobiology of rare intestinal cell types

Publications

• Pérez-González C, Ceada G, Greco F, Matejčić M, Gómez-González M, Castro M, Menendez A, Kale S, Krndija D, Clark AG, Gannavarapu RV, Alvarez-Varela A, Roca-Cusachs P, Batlle E, Matic Vignjevic D, Arroyo M, Trepat X.
  Mechanical compartmentalization of the intestinal organoid enables crypt folding and collective cell migration
  Nature Cell Biology
  2021 Jul
• Verhulsel M, Simon A, Bernheim-Dennery M, Gannavarapu VR, Geremie L, Ferraro D, Krndija D, Talini L, Viovy JL, Vignjevic DM.
  Developing an advanced gut on chip model enabling the study of epithelial cells/fibroblasts interactions
  Lab on a Chip
  2021 Jan
• El Marjou F, Jouhanneau C, Krndija D.
  Targeted Transgenic Mice Using CRISPR/Cas9 Technology
  Methods Mol Biol
  2021 Jan
• Lagoutte E, Villeneuve C, Fraisier V, Krndija D, Deugnier MA, Chavrier P, Rossé C.
  A new pipeline for pathophysiological analysis of the mammary gland based on organoid transplantation and organ clearing.
  J Cell Sci
  2020 Jun
• Krndija D*, Fairhead M.
  IGF1R undergoes active and directed centripetal transport on filopodia upon receptor activation.
  Biochem J
  2019 Dec *corresponding author

View all publications

Funding

         

Alumni

Chloé Jessu, M1 student (Jan-Apr 2023)
Baptiste Vigier, M1 student (Jan-Mar 2022)
Salomé Neuvendel, M2 student (Jan-Jun 2022)