Our team studies how the genetic information is organized in the intranuclear space and how this spatial organization contributes to regulate gene expression.
Our model organism is the yeast Saccharomyces cerevisiae, and we focus on the early stages of ribosome biogenesis. The precursor of the major ribosomal RNAs produced by the RNA polymerase I initiates the assembly of the nucleolus, the largest nuclear compartment; the nucleolus is the nuclear body in which early pre-ribosomal particles assembly is achieved.
By combining quantitative tools of cell biology, biochemistry and global genetic screens, we identify the molecular components that contribute to intra-nuclear architecture. We try to identify organizational principles that dictate the spatial organization of eukaryotic genomes.
Dauban L, Montagne R, Thierry A, Lazar-Stefanita L, Gadal O, Cournac A, Koszul R, Beckouët F. Regulation of cohesin-mediated mitotic chromosome folding by Eco1 and other partners Molecular cell 2020 Jan
Dauban L, Kamgoue A, Wang R, Leger-Silvestre I, Beckouët F, Cantaloube S, Gadal O. . Quantification of the dynamic behaviour of ribosomal DNA genes and nucleolus during yeast Saccharomyces cerevisiae cell cycle J. Struct. Biol 2019 Aug
Morlot S, Jia S, Léger-Silvestre I, Matifas A, Gadal O, Charvin G. Excessive rDNA Transcription Drives the Disruption in Nuclear Homeostasis during Entry into Senescence in Budding Yeast Cell report 2019 Jul
Socol M, Wang R, Jost D, Carrivain P, Vaillant C, Le Cam E, Dahirel V, Normand C, Bystricky K, Victor J-M, Gadal O, Bancaud, A,. Rouse model with transient intramolecular contacts on a timescale of seconds recapitulates folding and fluctuation of yeast chromosomes Nucleic Acids Res 2019 Jul
Walters AD, Amoateng K, Wang R, Chen JH, McDermott G, Larabell CA, Gadal O, Cohen-Fix O. Nuclear envelope expansion in budding yeast is independent of cell growth and does not determine nuclear volume Mol Biol Cell 2019 Jan