Dynamic nuclear organization

Olivier Gadal

Frederic Beckouet


CR CNRS
05 61 33 59 39

CR CNRS
05 61 33 58 10

Team members

  Researcher
  • Frederic Beckouet
  • Christophe Dez
  • Olivier Gadal
  Associate professor
  • Isabelle Leger-Silvestre
  Technical staff
  • Sophie Queille
  PhD student
  • Henri Mboumba
  • Nathalie Bastie
  • Claudie Carron
  • Chaima Azouzi
  Master student
  • Sarah Danche

Presentation

Our team studies how the genetic information is organized in the intranuclear space and how this spatial organization contributes to regulate gene expression.

Our model organism is the yeast Saccharomyces cerevisiae, and we focus on the early stages of ribosome biogenesis. The precursor of the major ribosomal RNAs produced by the RNA polymerase I initiates the assembly of the nucleolus, the largest nuclear compartment; the nucleolus is the nuclear body in which early pre-ribosomal particles assembly is achieved.

By combining quantitative tools of cell biology, biochemistry and global genetic screens, we identify the molecular components that contribute to intra-nuclear architecture. We try to identify organizational principles that dictate the spatial organization of eukaryotic genomes.

Projets

Study of cohesin complex in vivo

VISUALIZATION OF GENE TRANSCRIPTION AT SINGLE-MOLECULE RESOLUTION

Regulation of ribosomal RNA synthesis by RNA polymerase I

Visualization of chromatin loops in vivo

Publications

  • Bastié N, Chapard C, Dauban L, Gadal O, Beckouët F, Koszul R..
    Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops
    Nat Struct Mol Biol
    2022 Jun
  • Chaima Azouzi , Mariam Jaafar , Christophe Dez , Raghida Abou Merhi, Annick Lesne, Anthony K Henras, Olivier Gadal .
    Coupling Between Production of Ribosomal RNA and Maturation: Just at the Beginning
    Front Mol Biosci
    2021 Oct
  • Lesage E, Perez-Fernandez J, Queille S, Dez C, Gadal O, Kwapisz M..
    Non-Coding, RNAPII-Dependent Transcription at the Promoters of rRNA Genes Regulates Their Chromatin State in S. cerevisiae.
    Noncoding RNA.
    2021 Jul ODN
  • Okuda EK, Gonzales-Zubiate FA, Gadal O, Oliveira CC. .
    Nucleolar localization of the yeast RNA exosome subunit Rrp44 hints at early pre-rRNA processing as its main function.
    J. Biol Chem.
    2020 Aug
  • Dauban L, Montagne R, Thierry A, Lazar-Stefanita L, Gadal O, Cournac A, Koszul R, Beckouët F.
    Regulation of cohesin-mediated mitotic chromosome folding by Eco1 and other partners
    Molecular cell
    2020 Jan

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Funding

       

Alumni

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Université Paul Sabatier
118 Route de Narbonne

31062 TOULOUSE Cedex
France

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