Our team studies how the genetic information is organized in the intranuclear space and how this spatial organization contributes to regulate gene expression.
Our model organism is the yeast Saccharomyces cerevisiae, and we focus on the early stages of ribosome biogenesis. The precursor of the major ribosomal RNAs produced by the RNA polymerase I initiates the assembly of the nucleolus, the largest nuclear compartment; the nucleolus is the nuclear body in which early pre-ribosomal particles assembly is achieved.
By combining quantitative tools of cell biology, biochemistry and global genetic screens, we identify the molecular components that contribute to intra-nuclear architecture. We try to identify organizational principles that dictate the spatial organization of eukaryotic genomes.
Bastié N, Chapard C, Dauban L, Gadal O, Beckouët F, Koszul R.. Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops Nat Struct Mol Biol 2022 Jun
Chaima Azouzi , Mariam Jaafar , Christophe Dez , Raghida Abou Merhi, Annick Lesne, Anthony K Henras, Olivier Gadal . Coupling Between Production of Ribosomal RNA and Maturation: Just at the Beginning Front Mol Biosci 2021 Oct
Okuda EK, Gonzales-Zubiate FA, Gadal O, Oliveira CC. . Nucleolar localization of the yeast RNA exosome subunit Rrp44 hints at early pre-rRNA processing as its main function. J. Biol Chem. 2020 Aug
Dauban L, Montagne R, Thierry A, Lazar-Stefanita L, Gadal O, Cournac A, Koszul R, Beckouët F. Regulation of cohesin-mediated mitotic chromosome folding by Eco1 and other partners Molecular cell 2020 Jan