Dynamic nuclear organization

Olivier Gadal


CR CNRS
05 61 33 59 39

Team members

  Researcher
  • Frederic Beckouet
  • Christophe Dez
  • Olivier Gadal
  Associate professor
  • Isabelle Leger-silvestre
  PhD student
  • Nathalie Bastie

Presentation

Our team studies how the genetic information is organized in the intranuclear space and how this spatial organization contributes to regulate gene expression.

Our model organism is the yeast Saccharomyces cerevisiae, and we focus on the early stages of ribosome biogenesis. The precursor of the major ribosomal RNAs produced by the RNA polymerase I initiates the assembly of the nucleolus, the largest nuclear compartment; the nucleolus is the nuclear body in which early pre-ribosomal particles assembly is achieved.

By combining quantitative tools of cell biology, biochemistry and global genetic screens, we identify the molecular components that contribute to intra-nuclear architecture. We try to identify organizational principles that dictate the spatial organization of eukaryotic genomes.

Projets

How is condensin recruited onto ribosomal DNA?

VISUALIZATION OF GENE TRANSCRIPTION AT SINGLE-MOLECULE RESOLUTION

Genetic tools to study nucleolus

Biophysical characterization of chromatin in yeast cell's nucleolus

Publications

  • Dauban L, Montagne R, Thierry A, Lazar-Stefanita L, Gadal O, Cournac A, Koszul R, Beckouët F.
    Regulation of cohesin-mediated mitotic chromosome folding by Eco1 and other partners
    Molecular cell
    2020 Jan
  • Dauban L, Kamgoue A, Wang R, Leger-Silvestre I, Beckouët F, Cantaloube S, Gadal O. .
    Quantification of the dynamic behaviour of ribosomal DNA genes and nucleolus during yeast Saccharomyces cerevisiae cell cycle
    J. Struct. Biol
    2019 Aug
  • Morlot S, Jia S, Léger-Silvestre I, Matifas A, Gadal O, Charvin G.
    Excessive rDNA Transcription Drives the Disruption in Nuclear Homeostasis during Entry into Senescence in Budding Yeast
    Cell report
    2019 Jul
  • Socol M, Wang R, Jost D, Carrivain P, Vaillant C, Le Cam E, Dahirel V, Normand C, Bystricky K, Victor J-M, Gadal O, Bancaud, A,.
    Rouse model with transient intramolecular contacts on a timescale of seconds recapitulates folding and fluctuation of yeast chromosomes
    Nucleic Acids Res
    2019 Jul
  • Albert B, Kos-Braun IC, Henras AK, Dez C, Rueda M-P, Zhang X, Gadal O, Kos M, Shore M. .
    A ribosome assembly stress response regulates transcription to maintain proteome homeostasis
    Elife
    2019 May

View all publications

Funding

 

IDEX Toulouse (CLEMGENE et NUDGENE), ANR-ANDY

Alumni

https://www-lbme.biotoul.fr/uk/equipes/grpgadal/alumni.html

Institute

Université Paul Sabatier
118 Route de Narbonne

31062 TOULOUSE Cedex
France

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