Dynamic nuclear organization

Olivier Gadal

Frederic Beckouet


CR CNRS
05 61 33 59 39

CR CNRS
05 61 33 58 10

Team members

  Researcher
  • Frederic Beckouet
  • Christophe Dez
  • Olivier Gadal
  Associate professor
  • Isabelle Leger-Silvestre
  Technical staff
  • Sophie Queille
  PhD student
  • Nathalie Bastie
  • Claudie Carron
  Master student
  • Henri Mboumba

Presentation

Our team studies how the genetic information is organized in the intranuclear space and how this spatial organization contributes to regulate gene expression.

Our model organism is the yeast Saccharomyces cerevisiae, and we focus on the early stages of ribosome biogenesis. The precursor of the major ribosomal RNAs produced by the RNA polymerase I initiates the assembly of the nucleolus, the largest nuclear compartment; the nucleolus is the nuclear body in which early pre-ribosomal particles assembly is achieved.

By combining quantitative tools of cell biology, biochemistry and global genetic screens, we identify the molecular components that contribute to intra-nuclear architecture. We try to identify organizational principles that dictate the spatial organization of eukaryotic genomes.

Projets

Study of cohesin complex in vivo

VISUALIZATION OF GENE TRANSCRIPTION AT SINGLE-MOLECULE RESOLUTION

Regulation of ribosomal RNA synthesis by RNA polymerase I

Visualization of chromatin loops in vivo

Publications

  • Lesage E, Perez-Fernandez J, Queille S, Dez C, Gadal O, Kwapisz M..
    Non-Coding, RNAPII-Dependent Transcription at the Promoters of rRNA Genes Regulates Their Chromatin State in S. cerevisiae.
    Noncoding RNA.
    2021 Jul ODN
  • Okuda EK, Gonzales-Zubiate FA, Gadal O, Oliveira CC. .
    Nucleolar localization of the yeast RNA exosome subunit Rrp44 hints at early pre-rRNA processing as its main function.
    J. Biol Chem.
    2020 Aug
  • Dauban L, Montagne R, Thierry A, Lazar-Stefanita L, Gadal O, Cournac A, Koszul R, Beckouët F.
    Regulation of cohesin-mediated mitotic chromosome folding by Eco1 and other partners
    Molecular cell
    2020 Jan
  • Dauban L, Kamgoue A, Wang R, Leger-Silvestre I, Beckouët F, Cantaloube S, Gadal O. .
    Quantification of the dynamic behaviour of ribosomal DNA genes and nucleolus during yeast Saccharomyces cerevisiae cell cycle
    J. Struct. Biol
    2019 Aug
  • Morlot S, Jia S, Léger-Silvestre I, Matifas A, Gadal O, Charvin G.
    Excessive rDNA Transcription Drives the Disruption in Nuclear Homeostasis during Entry into Senescence in Budding Yeast
    Cell report
    2019 Jul

View all publications

Funding

     

IDEX Toulouse (CLEMGENE et NUDGENE), ANR-ANDY

Alumni

Liste of all alumni

Institute

Université Paul Sabatier
118 Route de Narbonne

31062 TOULOUSE Cedex
France

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