Multiscale Electron Imaging METi

Presentation

Multiscale Electron Imaging METi

The Multiscale Electron Imaging (METi) platform is a transmission electron microscopy facility within the Centre de Biologie Intégrative de Toulouse (CBI).

The METi takes part to the “Toulouse Réseau Imagerie” (TRI-IBiSA) cell imaging network of the Génopole Toulouse-Midi-Pyrénées and obtained the ISO 9001:2015 – NFX50-900 certification in January 2010.

MISSIONS

This facility is open to both academic and industrial users and offers three types of services. We can prepare samples, provide equipments, develop and manage projects. Our expertise is centered on biology, but projects in other fields (material science, chemistry, geology,…) have been successfully carried out.

The METi is especially involved in the implementation of new technologies designed to prepare and observe samples as close as possible to their native state:

• Cryo-electron microscopy / Single particles analysis
• Cryomethods : cryofixation, freeze substitution and cryosections
• (Cryo) Electron tomography

EQUIPMENTS

	TALOS ARCTICA TEM FEG 200 kV equipped with a DDE camera with post-column energy-filter Gatan K3-BioQuantum and phase plate
	JEOL 1400 TEM 120 kV equipped with CCD camera Gatan Orius SC1000B 11Mpixels
	JEOL 2100 TEM 200 kV equipped with a US1000 camera and a US4000 camera with post-column energy-filter Gatan GIF Quantum
	LEICA EMGP Automatic plunge freezer
	LEICA EM ICE High pressure freezer
	LEICA AFS2/FSP Automatic freeze substitution system
	LEICA UC7/FC7 Cryoultramicrotome to perform ultrathin section at room temperature or more specifically dedicated for cryosectionning for Tokuyasu or CEMOVIS technique

CONTACT

Funding