Molecular Chaperone Networks

Presentation

The Molecular Chaperone Networks research group at the Laboratory of Microbiology and Molecular genetics (LMGM-CBI) mainly focuses on the biology of molecular chaperone machines in bacteria. Genetics, molecular biology and biochemistry technics are used to identify new molecular chaperone functions and to pin point key interplay between major chaperones, such as DnaK/HSP70, DnaJ/HSP40, GroEL/HSP60, Trigger Factor or SecB, and other bacterial stress proteins, including Toxin-Antitoxin systems (see Figure). More recently, we have focused on SecB-like molecular chaperones in the human pathogen M. tuberculosis and revealed for the first time the control of a stress-responsive Toxin-Antitoxin system by molecular chaperones. 

Publications

• Yiming Cai, Ben Usher, Claude Gutierrez, Anastasia Tolcan, Moise Mansour, Peter C. Fineran, Ciarán Condon, Olivier Neyrolles, Pierre Genevaux, Tim R. Blower.
  A nucleotidyltransferase toxin inhibits growth of Mycobacterium tuberculosis through inactivation of tRNA acceptor stems
  Science Advances
  2020 Jul Vol. 6, no. 31, eabb6651 DOI: 10.1126/sciadv.abb6651
• Barriot R, Latour J, Castanié-Cornet MP, Fichant G, Genevaux P.
  J-Domain Proteins in Bacteria and Their Viruses
  J Mol Biol.
  2020 Apr S0022-2836(20)30301-6. doi:10.1016/j.jmb.2020.04.014
• Ayala S, Genevaux P, Hureau C, Faller P.
  (Bio)chemical Strategies To Modulate Amyloid-β Self-Assembly
  ACS Chem. Neurosci
  2019 Aug 10(8):3366-3374. doi:10.1021/acschemneuro.9b00239
• Akarsu H, Mansour M, Bordes P, Bigot DJ, Genevaux P, Falquet L.
  TASmania: a bacterial Toxin-Antitoxin Systems database
  PLoS Computational Biology
  2019 Apr
• Freire DM, Gutierrez C, Garza-Garcia A, Grabowska AD, Sala AJ, Ariyachaokun K, Panikova T, Beckham KSH, Colom A, Pogenberg V, Cianci M, Tuukkanen A, Boudehen YM, Peixoto A, Botella L, Svergun DI, Schnappinger D, Schneider TR, Genevaux P, de Carvalho LPS, Wilmanns M, Parret AHA and Neyrolles O.
  An NAD+ phosphorylase toxin triggers Mycobacterium tuberculosis cell death
  Mol. Cell
  2019 Mar

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Funding

           

Alumni

Moïse Mansour
Olivier Fayet
Marie-Françoise Prère
Donna Joe Bigot
Sarah Ayala
Ola Al-fawares
Pauline Texier
Nawel Slama
Ambre Sala
Frédéric Angles
Nicolas Bruel
Elsa Perrody