Molecular Chaperone Networks

Presentation

The Molecular Chaperone Networks research group at the Laboratory of Microbiology and Molecular genetics (LMGM-CBI) mainly focuses on the biology of molecular chaperone machines in bacteria. Genetics, molecular biology and biochemistry technics are used to identify new molecular chaperone functions and to pin point key interplay between major chaperones, such as DnaK/HSP70, DnaJ/HSP40, GroEL/HSP60, Trigger Factor or SecB, and other bacterial stress proteins, including Toxin-Antitoxin systems (see Figure). More recently, we have focused on SecB-like molecular chaperones in the human pathogen M. tuberculosis and revealed for the first time the control of a stress-responsive Toxin-Antitoxin system by molecular chaperones. 

Publications

• Zhao L, Castanié-Cornet MP, Kumar S, Genevaux P, Hayer-Hartl M, Hartl FU..
  Bacterial RF3 senses chaperone function in co-translational folding
  Mol. Cell.
  2021 Jun S1097-2765(21)00400-7. doi: 10.1016/j.molcel.2021.05.016. PMID: 34107307
• Bordes P, Genevaux P..
  Control of Toxin-Antitoxin Systems by Proteases in Mycobacterium Tuberculosis
  Front. Mol. Biosci.
  2021 May 8:691399. doi: 10.3389/fmolb.2021.691399. eCollection 2021. PMID: 34079824 (Review)
• Fauvet B, Finka A, Castanié-Cornet MP, Cirinesi AM, Genevaux P, Quadroni M, Goloubinoff P..
  Bacterial Hsp90 Facilitates the Degradation of Aggregation-Prone Hsp70-Hsp40 Substrates
  Front. Mol. Biosci.
  2021 Apr 8:653073. doi: 10.3389/fmolb.2021.653073. PMID: 33937334
• Texier P, Bordes P, Nagpal J, Sala AJ, Mansour M, Cirinesi AM, Xu X, Dougan DA, Genevaux P.
  ClpXP-mediated Degradation of the TAC Antitoxin is Neutralized by the SecB-like Chaperone in Mycobacterium tuberculosis
  J Mol Biol. 433(5):166815. doi: 10.1016/j.jmb.2021.166815
  2021 Jan Online ahead of print. PMID: 33450247
• Yiming Cai, Ben Usher, Claude Gutierrez, Anastasia Tolcan, Moise Mansour, Peter C. Fineran, Ciarán Condon, Olivier Neyrolles, Pierre Genevaux, Tim R. Blower.
  A nucleotidyltransferase toxin inhibits growth of Mycobacterium tuberculosis through inactivation of tRNA acceptor stems
  Science Advances
  2020 Jul Vol. 6, no. 31, eabb6651 DOI: 10.1126/sciadv.abb6651

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Funding

           

Alumni

Moïse Mansour
Olivier Fayet
Marie-Françoise Prère
Donna Joe Bigot
Sarah Ayala
Ola Al-fawares
Pauline Texier
Nawel Slama
Ambre Sala
Frédéric Angles
Nicolas Bruel
Elsa Perrody