RNA biology in Archaea

Béatrice Clouet-d´orval

05 61 33 58 75

Team members

  Researcher  Technical staff  PhD student
  • Manon Batista
  • Clarisse Etienne
  • Mirna Hajj
  Master student
  • Julie Caumes



B. Clouet-d’Orval was appointed as a group leader of the “RNA biology in Archaea” project by the Scientific Committee of the CBI following the 2018 Emerging team Call


The team “RNA biology in Archaea”, in close collaboration with local, national and international partners aims at identifying and characterizing RNA metabolic pathways that are at the centre of gene expression regulation in Euryarchaea. We develop three interconnected, basic research projects to identify and characterize novel enzymes and machineries that mediate RNA quality control, RNA stability and RNA processing pathways using Thermococcales and Halophiles as study models:

-The first project focus is to decipher the functions of archaeal β-CASP RNases and associated RNA helicases and to identify RNA metabolic pathways at work in archaeal cells (ANR-CASPAR project, BCO coordinator).

-The second focus is to elucidate how the RNA and protein quality control are coupled and coordinated to fine-tune gene expression (ANR-PROTEIN Project, BCO partner).

-Finally, the third focus is to shed light on the molecular mechanisms that control the initial steps of RNA decay.

We believe that our work will provide knowledge that should prove to be milestones in the fields of RNA metabolism and molecular biology of Archaea, the third domain of life, and potentially clarify corresponding mechanisms in Eukarya and Bacteria. In addition, it should enlighten us on specific molecular processes that allow the archaeal cells to sustain an extremophile lifestyle.


Initial steps on mRNA decay in Thermococcales

Connection between the ribosome and the RNA modification/degradation machineries in Thermococcales

Impact of the universally conserved ribonucleases from the β-CASP family on archaeal RNA metabolism


  • Manon Batista1, Petra Langendijk-Genevaux1, Mirna Hajj1,Duy Khanh Phung2, Laura Plassart3, Marie Bouvier1, Gwennaële Fichant1, and Béatrice Clouet-d’Orval1*.
    The Archaeal Specific Helicase of the Ski2-like family, ASH-Ski2, is an authentic RNA helicase
    2019 Dec en préparation
  • Duy Khanh Phung, Clarisse Etienne, Manon Batista, Petra Langendijk-Genevaux, Yann Moalic, Sébastien Laurent, Violette Morales, Mohamed Jebbar, Gwennaele Fichant, Marie Bouvier, Didier Flament, Béatrice Clouet-d’Orval.
    RNA processing machineries in Archaea: the 5′-3′ exoribonuclease aRNase J of the β-CASP family is engaged specifically with the helicase ASH-Ski2 and the 3′-5′ exoribonucleolytic RNA exosome machinery
    2019 Jul
  • Hajj M., El-Hamaoui, S. Batista M.,Bouvier M., Abdel-Razzak, Z, Clouet-D’Orval, B*., and Chamieh, H.
    Archaeal SF1 and SF2 helicases: Unwinding in the extreme
    Helicases from all domains of life edited by Dr. Tutejah, Acadamic Press Elsevier,
    2018 Dec Chap 1 pp 1-17 ISBN: 978-0-12-814685-9
  • Hogrel G., Lu Y., Laurent S., Henry E., Phung DK., Bossé A., Pluchon P. F., Clouet-d’Orval B. and Flament D. .
    Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus
    Nucleic Acids Research,
    2018 May vol 46 pp 5651-5663-doi: 10.1093/nar/gky322
  • Clouet-d'Orval B*, Batista M, Bouvier M, Quentin Y, Fichant G, Marchfelder A, Maier LK.
    Insights into RNA processing pathways and associated-RNA degrading enzymes in Archaea.
    FEMS Microbiol Rev.
    2018 Apr DOI:10.1093/femsre/fuy016

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Duy Khanh Phung

Dana Rinaldi

Bashir Saadeh

Kounthéa Phok


Université Paul Sabatier
118 Route de Narbonne

31062 TOULOUSE Cedex

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