Biologie de l'ARN chez les Archées

Présentation

SYNOPSIS

B. Clouet-d’Orval was appointed as a group leader of the “RNA biology in Archaea” project by the Scientific Committee of the CBI following the 2018 Emerging team Call

FOCUSES

The team “RNA biology in Archaea”, in close collaboration with local, national and international partners aims at identifying and characterizing RNA metabolic pathways that are at the centre of gene expression regulation in Euryarchaea. We develop three interconnected, basic research projects to identify and characterize novel enzymes and machineries that mediate RNA quality control, RNA stability and RNA processing pathways using Thermococcales and Halophiles as study models:

-The first project focus is to decipher the functions of archaeal β-CASP RNases and associated RNA helicases and to identify RNA metabolic pathways at work in archaeal cells (ANR-CASPAR project, BCO coordinator).

-The second focus is to elucidate how the RNA and protein quality control are coupled and coordinated to fine-tune gene expression (ANR-PROTEIN Project, BCO partner).

-Finally, the third focus is to shed light on the molecular mechanisms that control the initial steps of RNA decay.

We believe that our work will provide knowledge that should prove to be milestones in the fields of RNA metabolism and molecular biology of Archaea, the third domain of life, and potentially clarify corresponding mechanisms in Eukarya and Bacteria. In addition, it should enlighten us on specific molecular processes that allow the archaeal cells to sustain an extremophile lifestyle.

Projets

Molecular mechanisms of the 5’-3’ RNA degradation in Thermococcales

Coupling between the RNA & protein decay machineries in Thermococcales

Impact of the universally conserved ribonucleases from the β-CASP family on archaeal RNA metabolism

Publications

• Manon Batista1, Petra Langendijk-Genevaux1, Mirna Hajj1,Duy Khanh Phung2, Laura Plassart3, Marie Bouvier1, Gwennaële Fichant1, and Béatrice Clouet-d’Orval1*.
  The Archaeal Specific Helicase of the Ski2-like family, ASH-Ski2, is an authentic RNA helicase
  2019 Dec en préparation
• Duy Khanh Phung, Clarisse Etienne, Manon Batista, Petra Langendijk-Genevaux, Yann Moalic, Sébastien Laurent, Violette Morales, Mohamed Jebbar, Gwennaele Fichant, Marie Bouvier, Didier Flament, Béatrice Clouet-d’Orval.
  RNA processing machineries in Archaea: the 5′-3′ exoribonuclease aRNase J of the β-CASP family is engaged specifically with the helicase ASH-Ski2 and the 3′-5′ exoribonucleolytic RNA exosome machinery
  BIORXIV/2019/699629
  2019 Jul
• Hajj M., El-Hamaoui, S. Batista M.,Bouvier M., Abdel-Razzak, Z, Clouet-D’Orval, B*., and Chamieh, H.
  Archaeal SF1 and SF2 helicases: Unwinding in the extreme
  Helicases from all domains of life edited by Dr. Tutejah, Acadamic Press Elsevier,
  2018 Dec Chap 1 pp 1-17 ISBN: 978-0-12-814685-9
• Hogrel G., Lu Y., Laurent S., Henry E., Phung DK., Bossé A., Pluchon P. F., Clouet-d’Orval B. and Flament D. .
  Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus
  Nucleic Acids Research,
  2018 May vol 46 pp 5651-5663-doi: 10.1093/nar/gky322
• Clouet-d'Orval B*, Batista M, Bouvier M, Quentin Y, Fichant G, Marchfelder A, Maier LK.
  Insights into RNA processing pathways and associated-RNA degrading enzymes in Archaea.
  FEMS Microbiol Rev.
  2018 Apr DOI:10.1093/femsre/fuy016

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Financements

       

Anciens membres

Duy Khanh Phung

Dana Rinaldi

Bashir Saadeh

Kounthéa Phok

Laura Plassart