Biologie de l'ARN chez les Archées

Béatrice Clouet D´orval

05 61 55 69 06

Membres de l'équipe

  Ingénieur de recherches
  • Régine Capeyrou
  Chercheur  Enseignant-chercheur
  • Manon Batista
  Soutien technique
  • Isabelle Canal



Béa Clouet-d’Orval is the a group leader of the RNARCHAEA “RNA biology of Archaea” team. Béa Clouet-d’Orval & Marie Bouvier are co-supervizing the scientific projects of the team.


The team “RNA biology of Archaea”, in close collaboration with local, national and international partners aims at identifying and characterizing RNA metabolic pathways that are at the centre of gene expression regulation in Euryarchaea. We develop three interconnected, basic research projects to identify and characterize novel enzymes and machineries that mediate RNA quality control, RNA stability and RNA processing pathways using Thermococcales and Halophiles as study models:

-The first project focus is to decipher the functions of archaeal β-CASP RNases and associated RNA helicases and to identify RNA metabolic pathways at work in archaeal cells (ANR-CASPAR project, BCO coordinator).

-The second focus is to elucidate how the RNA and protein quality control are coupled and coordinated to fine-tune gene expression (ANR-PROTEIN Project, BCO partner).

-Finally, the third focus is to shed light on the molecular mechanisms that control the initial steps of RNA decay.

We believe that our work will provide knowledge that should prove to be milestones in the fields of RNA metabolism and molecular biology of Archaea, the third domain of life, and potentially clarify corresponding mechanisms in Eukarya and Bacteria. In addition, it should enlighten us on specific molecular processes that allow the archaeal cells to sustain an extremophile lifestyle.


Molecular mechanisms of the 5’-3’ RNA degradation in Thermococcales

Coupling between the RNA & protein decay machineries in Thermococcales

Impact of the universally conserved ribonucleases from the β-CASP family on archaeal RNA metabolism


  • Manon Batista1, Petra Langendijk-Genevaux1, Mirna Hajj1,Duy Khanh Phung2, Laura Plassart3, Marie Bouvier1, Gwennaële Fichant1, and Béatrice Clouet-d’Orval1*.
    The Archaeal Specific Helicase of the Ski2-like family, ASH-Ski2, is an authentic RNA helicase
    2019 Dec en préparation
  • Duy Khanh Phung, Clarisse Etienne, Manon Batista, Petra Langendijk-Genevaux, Yann Moalic, Sébastien Laurent, Violette Morales, Mohamed Jebbar, Gwennaele Fichant, Marie Bouvier, Didier Flament, Béatrice Clouet-d’Orval.
    RNA processing machineries in Archaea: the 5′-3′ exoribonuclease aRNase J of the β-CASP family is engaged specifically with the helicase ASH-Ski2 and the 3′-5′ exoribonucleolytic RNA exosome machinery
    2019 Jul
  • Hajj M., El-Hamaoui, S. Batista M.,Bouvier M., Abdel-Razzak, Z, Clouet-D’Orval, B*., and Chamieh, H.
    Archaeal SF1 and SF2 helicases: Unwinding in the extreme
    Helicases from all domains of life edited by Dr. Tutejah, Acadamic Press Elsevier,
    2018 Dec Chap 1 pp 1-17 ISBN: 978-0-12-814685-9
  • Hogrel G., Lu Y., Laurent S., Henry E., Phung DK., Bossé A., Pluchon P. F., Clouet-d’Orval B. and Flament D. .
    Physical and functional interplay between PCNA DNA clamp and Mre11–Rad50 complex from the archaeon Pyrococcus furiosus
    Nucleic Acids Research,
    2018 May vol 46 pp 5651-5663-doi: 10.1093/nar/gky322
  • Clouet-d'Orval B*, Batista M, Bouvier M, Quentin Y, Fichant G, Marchfelder A, Maier LK.
    Insights into RNA processing pathways and associated-RNA degrading enzymes in Archaea.
    FEMS Microbiol Rev.
    2018 Apr DOI:10.1093/femsre/fuy016

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Anciens membres

Mirna Hajj- former PhD

Manon Batista- former PhD

Duy Khanh Phung- former PhD

Bashir Saadek- former Post-doc

Dana Rinaldi

Laura Plassart

Université Paul Sabatier
118 Route de Narbonne

31062 TOULOUSE Cedex

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