Role of cell cycle in cell fate decision
Fabienne Pituello
DR CNRS
05 61 55 83 49
Team members
Researcher- Eric Agius
- Sophie Bel-Vialar
- Fabienne Pituello
Associate professor Technical staff- Francois Médevielle
- Perrine Uhart
PhD student- Sofiane Omerani
- Delphine Bretonniere
- Julie Pignolet
Presentation
Our aim is to understand how the cell cycle affects cell fate decisions in organ development and homeostasis using as a paradigm the developing nervous system. Deciphering the core machinery of cell cycle has been mostly the focus of cell biologists, while, developmental biologists identified the signaling pathways and transcriptional programs controlling cell fate choices. The interplay between these two fundamental aspects of biology remained largely unexplored. The goal of our project is to implement molecular analyses with advanced technique in time-lapse imaging to elucidate how changes in cell cycle features switch a proliferating neural progenitor into a differentiating neuron.
Projets
Function of a cell cycle regulator, the CDC25B phosphatase, in Vertebrate neurogenesis
Role of cell cycle in cell fate decision during organogenesis: Insight into the developing nervous system using single cell high resolution time lapse imaging
The CDC25B phosphatase shortens the G2 phase of neural progenitors and promotes efficient neuron production
The proneural NeuroG2 drives cell cycle exit of neuronal precursors by specifically repressing a subset of cyclins acting at the G1 and S phases of the cell cycle
Publications
- Angie Molina, Frederic Bonnet, Julie Pignolet, Valerie Lobjois, Sophie Bel-vialar, Jacques Gautrais, Fabienne Pituello, Eric Agius.
Single-cell imaging of the cell cycle reveals CDC25B-induced heterogeneity of G1 phase length in neural progenitor cells
Development
2022 Jun - Bonnet F, Molina A, Roussat M, Azais M, Bel-Vialar S, Gautrais J, Pituello F, Agius E.
Neurogenic decisions require a cell cycle independent function of the CDC25B phosphatase.
Elife
2018 Jul - Lacomme M, Medevielle F, Bourbon HM, Thierion E, Kleinjan DJ, Roussat M, Pituello F, Bel-Vialar S..
A long range distal enhancer controls temporal fine-tuning of PAX6 expression in neuronal precursors.
Dev Biol.
2018 Apr - Angie Molina, Fabienne Pituello.
Playing with the cell cycle to build the spinal cord
Developmental Biology
2017 Dec review - 2015 Jan AGIUS, E., BEL-VIALAR, S., BONNET, F., PITUELLO, F. (2015). Cell cycle and cell fate in the developing nervous system: the role of CDC25B phosphatase. Cell Tissue Res 359, 201-13.
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