Developmental Regulation of Gene Dosage

Presentation

To find out more: www.galupalab.org

The goal of the lab is to decipher molecular mechanisms that help cells “do maths” during development – after all, cells are able to interpret numerical information, like the number of chromosomes in their nucleus or the gradients of morphogens in the extracellular space, and then “make” decisions based on that, such as which cell types to become. In particular, the lab focuses on how cells sense and interpret differential gene dosage, i.e., the number of copies of a gene in the genome. This includes studying the mammalian X chromosome, which is most often present in either one or two copies in the nucleus; this difference has had important implications during the evolution of mammals. As model systems, the lab mostly uses mouse embryonic stem cells and mouse embryos, following the ethical considerations associated with them.

Do not hesitate to reach out if you are interested in our research!

The calico cat image on the left is a drawing by Jenn Kay.

Picture from February 2024: Benoit, Estelle, Daniela and Rafael

Picture from October 2023: Daniela, Rafael and Benoit

Publications

• Galupa R#, Alvarez-Canales G*, Borst NO*, Fuqua T*, Gandara L*, Misunou N*, Richter K*, Alvez MRP, Karumbi E, Perkins ML, Kocijan T, Rushlow CA and Crocker J#..
  Enhancer architecture and chromatin accessibility constrain phenotypical space during development
  Developmental Cell
  2023 Jan #Corresponding authors
• Gandara L*, Tsai A*, Ekelöf M, Galupa R, Noon EPB, Alexandrov T and Crocker J.
  Developmental phenomics suggests that H3K4 monomethylation catalyzed by Trr functions as a phenotypic capacitor
  Cell Reports
  2022 Dec
• Hauth A, Galupa R, Servant N, Villacorta L, Hauschulz K, van Bemmel JG, Loda A and Heard E.
  Deciphering high-resolution 3D chromatin organization via Capture Hi-C
  JoVE
  2022 Oct
• Galupa R*#, Picard C*, Servant N, Nora EP, Zhan Y, van Bemmel JG, Zhan Y, El Marjou F, Johanneau C, Borensztein M, Ancelin K, Giorgetti L and Heard E#.
  Inversion of a topological domain leads to restricted changes in its gene expression and affects inter-domain communication
  Development
  2022 May *Equal contribution, #Corresponding authors

Funding