Agenda

Jacques Côté Université Laval, Québec, Canada
CODE séminaire public
Titre : "crosstalks between chromatin modifying activities"
11:00
4R4-RDC | Salle Conférence (182 pers.)

Léo ROLLAND & Elodie MITRI
CELLDYN séminaire interne
Titre : "p27kip1 controls cell metabolism " & "Regulation of Citron Kinase and importance in the glioblastoma stem cells "
11:00
4R4-RDC | Salle Conférence (182 pers.)

David OHAYON (Davy-MCD)
NEUROBIOLOGY séminaire interne
Titre : Astrocyte diversity: looking at a specific subpopulation, the Olig2-astrocytes
12:15
4R4-RDC | Salle Conférence (182 pers.)

Tung Le (John Innes Centre, Norwich)
SYSMIC séminaire public
Titre : Molecular switching of a DNA-sliding clamp to a repressor mediates long-range gene silencing on a multi-drug resistance plasmid
15:00
4R4-RDC | Salle Conférence (182 pers.)

Long-range gene regulation is rare in bacteria, and it remains unknown whether other mechanisms, beyond classical DNA looping, are involved. Here, we use a range of approaches, including crystallography and single-molecule analysis, to report that long-range gene silencing on the plasmid RK2, a source of multidrug resistance across diverse Gram-negative bacteria, is achieved cooperatively by a DNA-sliding clamp, KorB, and a clamp-locking protein, KorA. We show that KorB is a CTPase clamp that can entrap and slide along DNA to reach distal target promoters. We resolved the tripartite crystal structure of a KorB-KorA-DNA co-complex, finding that KorA latches KorB into a closed-clamp state. We argue that KorA-KorB interaction stalls KorB sliding at target core promoter elements to occlude RNA polymerase holoenzymes and thereby repress transcription. KorA-KorB interaction also enhances the residence time of the KorAB repressome at the target promoter, prolonging repression. Overall, our data uncovers the mechanism by which the clamp-locking protein KorA promotes a KorB functional switch between DNA sliding, which is important for plasmid segregation, and stalling, which is essential for KorB to mediate long-range repression.

Autre séminaire
Titre : RNA'OCC CLUB RNA OCCITANIE (8 eme Colloque)
09:45
4R4-RDC | Salle Conférence (182 pers.)

Conférence
Titre : RNA'OCC
08:30
4R4-RDC | Hall d'entrée

Conférence
Titre : RNA'OCC
09:30
Grilles

Denis KRNDIJA
Autre séminaire
Titre : FBI - Mechanobiology meeting
14:00
4R4-RDC | Salle Conférence (182 pers.)

RNA séminaire public
Titre : PICS RNA external
11:00
4R4-RDC | Salle Conférence (182 pers.)

Carine Rossé Institut Curie / Institut Jacques Monod,
CBI séminaire public
Titre : “Good Cop, Bad Cop: Mechanical Competition and Synergy in Breast Cancer Heterogeneity and Tumor Progression”
11:00
4R4-RDC | Salle Conférence (182 pers.)

CODE séminaire public
Titre : Séminaire PICS CODE
15:30
IBCG | Salle Conférence (100 pers.)