The Drosophila immune defense relies upon synthesis of anti-microbial peptides and activity of circulating cells, the hemocytes. Larval hematopoiesis takes place in a specialized organ, the lymph gland (LG). Our lab discovered a few years ago that Col was required for specification of the Posterior Signalling Center (PSC) in the LG and the production of lamellocytes, a cryptic hemocyte type, upon wasp parasitism. The PSC was then proposed to control, in a non cell autonomous manner, hemocyte homeostasis in the LG, reminiscent of the vertebrate hematopoietic niche in the bone marrow. Transcriptome analyses of LGs in col mutants and following parasitism (unpublished) revealed the role of JAK-STAT signalling in pro-hemocyte and BMP and Robo signaling in controlling PSC size and morphology. While the PSC contributes to LG homeostasis by regulating blood cell differentiation, col cell autonomously maintains a core population of LG progenitors. Lineage tracing further indicated that the hematopoietic progenitor population is heterogenous.
Our current projects focus on identifying parameters of prohemocyte plasticity, including the role of the vascular system, and how the PSC controls the cellular immune response to parasitism. Genetic manipulation of different cell types in the LG is expected to provide novel insight into the communication between the niche(s) and hematopoietic stem cells and progenitors in the mammalian bone marrow.
Recent publications : Morin-Poulard, I., Sharma, A., Louradour, I., Vanzo, N., Vincent, A. and Crozatier, M. Nature communications. 2016
Reactive oxygen species-dependent Toll/NF-κB activation in the Drosophila hematopoietic niche confers resistance to wasp parasitism I. Louradour, A. Sharma, I. Morin-Poulard, M. Letourneau, A. Vincent, M. Crozatier and N. Vanzo . eLIFE 2017
Textbook drawings of human anatomy illustrate the morphological diversity of body muscles allowing precision and strength of movements. Each Drosophila skeletal muscle displays a specific morphological identity (size, shape, orientation, innervation) and forms by fusion of a Founder Cell (FC) with naïve myoblasts. FCs originate from muscle progenitor cells (PCs). Our laboratory contributed to show that muscle morphological identity reflects the expression by each PC/FC of a specific combination of “identity” Transcription Factors, and integrates temporal, positional and homeotic information. Transcriptional identity is then propagated to the nuclei of fusing naive myoblasts, in parallel to implementation of muscle differentiation (Bataillé et al., 2017). We recently genetically identified new iTFs. This provided a novel, dynamic view of the transcriptional control of muscle identity, by combinatorial inputs from lineage-specific TFs, e.g., Col/EBF, Tup/Islet1, and general myogenic Transcription Factors, e.g., Eya, Six, Nau/MyoD (Dubois et al., 2016).
Ongoing work aims at identifying muscle identity realisator genes, using genome-wide profiling and computational approaches and studying the physiological consequences of specifc muscle pattern defects gnerated by genome editing.
Recent publications :
“Genetic dissection of the Transcription Factor code controlling serial specification of muscle identities in Drosophila“ Dubois et al., eLIFE 2016
"Dynamics of transcriptional (re)-programming of syncytial nuclei in developing muscles" Bataillé et al., BMC Biology 2017.
The Thoracic Alary-Related Muscles; A novel type of internal muscles.
In addition to body wall muscles, one Alary Muscle (AM) connects the exoskeleton to the heart in every abdominal segment. Our team recently discovered the existence of Thoracic Alary-Related Muscles (TARMS). TARMs appose to the respiratory system and connect visceral organs to the skeleton. Boukhatmi, H. , Schaub, C., Bataillé, L., Reim, I., Frendo J.L., Frasch, M.* and Vincent, A.*..An Org1/Tbx1-Tup/Islet1 transcriptional cascade reveals the existence of different types of alary muscles connecting internal organs in Drosophila. . Development, 2014
We propose that TARMs are novel type of muscles connnecting internal organs and playing an architectural role in sensing organ position ( similar to abseiling ropes).
A novel project of our lab is studying the development, function and innervation of TARMs, including live imaging and locomotion behavior tests, within and Evo/Devo framework.
Recent review : Bataille, L. et al., (2015) Mechanisms of Development, 138, 170-176.
Former PhD students ; present position
Manon LETOURNEAU, 2014-2018
Post-Doc, University of Montréal, Montréal, Canada, email@example.com
Alexandre CARAYON 2015-2018
Post-Doc, Department of Biomedical Science, University of Sheffield , UK
Isabelle LOURADOUR, 2012-2015
Post‐Doc, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, USA firstname.lastname@example.org
Ismaël MORIN-POULARD, 2011-2014
Post-Doc CBI, Toulouse email@example.com
Mathilde de TAFFIN, 2010-2013
Hadi BOUKHATMI, 2009-2012
Post-Doc, University of Cambridge, Cambridge, UK. firstname.lastname@example.org
Delphine PENNETIER, 2008-2011
Jonathan ENRIQUEZ, 2007-2010
PI, Development and function of the neuromuscular system, IGFL, Lyon
Rami MAKKI, 2006-2009
Post-Doc, The Francis Crick Institute, London, UK
Joanna KRZEMIEN 2006-2008
Project Manager, University of St Andrews, Scotland; email@example.com
Sébastien MELLA, 1999-2003
Bio-informatician, Institut Pasteur, Paris
Laurence DUBOIS 1995-1998
CNRS Research associate, Toulouse firstname.lastname@example.org
Abderhamann K’HILA, 1999-2002 (Co-supervision S. Ibnsouda, University Fes)
PI, Developmental genomics and evolution, IGFL, Lyon; email@example.com
Saad IbnSOUDA, 1991-1993, (+ Doctorat d’état 1997)
PI Directeur Laboratoire Biologie Microbienne, Université de Fes, Maroc
Stéphane NOSELLI, 1989-1992
Director, Institut Valrose, Nice
Valérie LEFRERE, 1989-1991 (Co-supervision F. Amalric, IBCG, Toulouse)
Assistant-Professor, LBAE, Auch, firstname.lastname@example.org
François PAYRE, 1987-1990, > 1988 (PhD + post-doc)
PI, Control of Cell Shape Remodeling, CBD Toulouse
François SCHWEISGUTH, 1987-1990 (Co-supervision, JA Lepesant, IJM Paris)
Nathalie COLOMBIé, 2017-2018
Engineer, digital technology company; email@example.com
Anurag SHARMA, 2013-2016
PI, Nitte University Centre for Science, Mangalore , India, firstname.lastname@example.org
Laetitia BATAILLE, 2010-2011
INSERM Research Associate, CBI, Toulouse email@example.com
Justine OYALLON, 2008-2011
Professor de Asignatura, Departamento de Biología Celular, Universidad Nacional Autónoma de México. firstname.lastname@example.org
Bruno GLISE, 2003-2005
Assistant Professor, University of Toulouse, Team Morphogens and Glial specification
François AGNES, 1987
Assistant Professor, University Paris Sud, Team Développement et Evolution du Cerveau Antérieur
Fabien CROZET, 1999-2000
CEO DPO Consulting Occitanie
Michel VERVOORT, 1997-1998
Denise VALLE 1994-1996
Deputy PI, Instituto Oswaldo Cruz and INCT-EM, Rio de Janeiro, Brasil, http://www.fiocruz.br/ioclabs/cgi/cgilua.exe/sys/start.htm?sid=37; email@example.com
Michèle CROZATIER, 1988-1990
Co-PI since 2002; firstname.lastname@example.org
Research Associates and Visiting Scientists
Pierre FERRER, 1988-2000
Maryvonne MEVEL-NINIO, 1994
Pasqualina BUONO, 1995
Professor, DSMB, University of Naples "Parthenope,", Naples, Italy.
Baya CHERIF-ZAHAR, 2010
ESPCI ParisTech-CNRS Paris, Team Plasticité du Cerveau