The Drosophila immune defense relies upon synthesis of anti-microbial peptides and activity of circulating cells, the hemocytes. Larval hematopoiesis takes place in a specialized organ, the lymph gland (LG). Our lab discovered a few years ago that Col was required for specification of the Posterior Signalling Center (PSC) in the LG and the production of lamellocytes, a cryptic hemocyte type, upon wasp parasitism. The PSC was then proposed to control, in a non cell autonomous manner, hemocyte homeostasis in the LG, reminiscent of the vertebrate hematopoietic niche in the bone marrow. Transcriptome analyses of LGs in col mutants and following parasitism (unpublished) revealed the role of JAK-STAT signalling in pro-hemocyte and BMP and Robo signaling (Nature Com., in press) in controlling PSC size and morphology. While the PSC contributes to LG homeostasis by regulating blood cell differentiation, col cell autonomously maintains a core population of LG progenitors. Lineage tracing further indicated that the hematopoietic progenitor population is heterogenous.
Our current projects focus on identifying parameters of prohemocyte plasticity, including the role of the vascular system, and how the PSC controls the cellular immune response to parasitism. Genetic manipulation of different cell types in the LG is expected to provide novel insight into the communication between the niche(s) and hematopoietic stem cells and progenitors in the mammalian bone marrow.
Textbook drawings of human anatomy illustrate the morphological diversity of body muscles allowing precision and strength of movements. Each Drosophila skeletal muscle displays a specific morphological identity (size, shape, orientation, innervation) and forms by fusion of a Founder Cells (FC) with naïve myoblasts. FCs originate from muscle progenitor cells (PCs) specified at fixed position and time within the somatic mesoderm. Our laboratory contributed to show that muscle identity reflects the expression by each PC/FC of a specific combination of “identity” transcription factors, and integrates temporal, positional and Homeotic information. Transcriptional identity is then propagated to recruited naive myoblasts upon fusion, in parallel to implementation of muscle differentiation (Bataillé et al., 2016, submitted). We recently genetically identified new iTFs. This provided a novel, dynamic view of the transcriptional control of muscle identity, including lineage-specific regulatory loops connecting evolutionarily conserved identity (Col/EBF, Tup/Islet1) and general myogenic Transcription Factors (Eya, Six Nau/MyoD (Dubois et al., 2016, under revision).
Ongoing work aims at identifying muscle identity realisator genes, using genome-wide profiling and computational approaches -TF and Cis-Regulatory Modules-, and understanding how the generic myogenic and muscle identity programs are coordinated to generate the stereotypical muscle pattern.
Article under revision (05 2016): “Integrating transcriptional dynamics of muscle identity and differentiation programs during Drosophila muscle development“
Article under revision (05 2016) “Genetic dissection of the Transcription Factor code controlling serial specification of muscle identities in Drosophila“
The Thoracic Alary-Related Muscles; A novel type of deformable muscles. (see new project)
Isabelle LOURADOUR 2012-2015, Post-doc, National Institute of Allergy and Infectious Diseases, NIH, Bethesda, USA
Ismaël MORIN-POULARD, 2011-2014. Post-Doc Hubrecht Institute, Utrecht, NL email@example.com
Mathilde de TAFFIN, 2010-2013. Catholic Sisterhood
Hadi BOUKHATMI, 2009-2012. Post-Doc, University of Cambridge, Cambridge, UK. firstname.lastname@example.org
Delphine PENNETIER, 2008-2011
Jonathan ENRIQUEZ, 2007-2009 Post-Doc, University of Columbia, NYC, USA. email@example.com
Rami MAKKI, 2005-2008. Post-Doc, The Francis Crick Institute, London, UK
Joanna KRZEMIEN 2006-2008. Project Manager, University of St Andrews, Scotland; firstname.lastname@example.org
Sébastien MELLA, 2002-2005
Abderhamann K’HILA, 2000-2003 (Co-supervision S. Ibnsouda, University Fes) PI, IGFL, Lyon; Developmental genomics and evolution. email@example.com>
Laurence DUBOIS, 1996-1999. CNRS Research associate, CBD Toulouse firstname.lastname@example.org
Cécile RUEZ, 1995-1997
Saad IbnSOUDA, 1991-1993, (+ Doctorat d’état 1997) Director Laboratoire Biologie Microbienne, Université de Fes, Maroc email@example.com
Stéphane NOSELLI, 1989-1992. Director, Institut Valrose, Nice; Epithelial Morphogenesis and left-right asymmetry in Drosophila.firstname.lastname@example.org
Valérie LEFRERE, 1989-1991 (Co-supervision F. Amalric, IBCG, Toulouse). Assistant-Professor, LBAE, Auch, email@example.com
François PAYRE, 1987-1990, (post-doc > 1997). PI, CBD Toulouse; Control of Cell Shape Remodeling, françois.firstname.lastname@example.org
François SCHWEISGUTH, 1987-1990 (Co-supervision, JA Lepesant, IJM Paris). PI, Drosophila Developmental Genetics, Pasteur Institute, Paris email@example.com
Anurag SHARMA, 2013-2016, Assistant Professeur, Dept.of Biological Sciences, Nitte University, Mangalore-575018, India
Laetitia BATAILLE, 2010-2011 INSERM Research Associate, firstname.lastname@example.org
Justine OYALLON, 2008-2011 Professor de Asignatura, Departamento de Biología Celular, Universidad Nacional Autónoma de México. email@example.com
Bruno GLISE, 2003-2005 Assistant Professor, University of Toulouse, Team Morphogens and Glial specification firstname.lastname@example.org
Fabien CROZET 2000-2001, Chief Information Officer and Head of Bioinformatics Department, MilleGen, Toulouse.
Michel VERVOORT, 1997-1999 Professor, PI, Metazoan Development and Evolution, Institut Jacques Monod, Paris Michel.VERVOORT@ijm.fr
Denise VALLE 1994-1996 Deputy PI, Instituto Oswaldo Cruz and INCT-EM, Rio de Janeiro, Brasil, email@example.com
Michèle CROZATIER, 1988-1990 Co-PI since 2002; firstname.lastname@example.org