Epigenetic circuits in living cells


  • Michela Anfossi
  • Fernando Muzzopappa

To understand how posttranslational histone modifications are associated with biological functions we introduce tunable histone-modifying enzymes into living cells and study their behavior. These enzymes are for example coupled to dCas9 so that they can be targeted to different genomic loci using different guide RNAs. One of our goals is to engineer circuits that consist of several of these enzymes, which act together to form complex functional modules like toggle switches or amplifiers. Based on such circuits we try to dissect the mechanisms underlying epigenetic phenomena.



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