Ségrégation active des génomes bactériens : mécanismes et diversité

Jean-yves Bouet


DR CNRS
05 61 33 59 06

Team members

  Researcher
  • François Boudsocq
  • Jean-yves Bouet
  • Catherine Guynet
  Associate professor
  • Franck Pasta
  Technical staff
  • Jérôme Rech
  PhD student
  • Valentin Quebre
  • Hicham Sekkouri-alaoui
  Master student
  • Romain Miranda-capet

Presentation

Our team studies the segregation of bacterial replicons to understand how bacteria ensure the faithful transmission of chromosomes and low copy number plasmids to daughter cells prior to cell division. Along this line, we develop three main research projects: (i) a molecular study of the partition of the F plasmid of Escherichia coli which constitutes a simple and autonomous model system. We aim to decipher the dynamic assemblies that self-organize and lead the movement and positioning of the different partners during the cell cycle, (ii) a study of DNA segregation in bacteria with complex genomes, such as P. naphtalenivorans, and (iii) a characterization of the interplay between bacterial segregation and conjugation. We aim to understand at the molecular level the determinants of mobile genetic elements that ensure vertical (segregation) and horizontal (conjugation) gene transfer.

 Reconstitution and modeling of all steps involved in DNA partitioning based on biochemical, genetics and live cell imaging will represent an important stage toward understanding the molecular mechanism of DNA segregation

Projets

Diversity of ParABS systems

Mechanisms of Bacterial DNA segregation

Interplay between bacterial segregation and conjugation: StbAB

Publications

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Funding

         

Institute

Université Paul Sabatier
118 Route de Narbonne

31062 TOULOUSE Cedex
France

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