Publications

Team publications GeDy (Bouet/Cornet)

Walter, J.-C., Rech, J., Walliser, N.-O., Dorignac, J., Geniet, F., Palmeri, J., Parmeggiani, A., and Bouet, J.-Y.
Physical modeling of a sliding clamp mechanism for the spreading of ParB at short genomic distance from bacterial centromere sites
iScience
2020 Dec 2020.101861
Sandra Daniel, Kelly Goldlust, Valentin Quebre, Minjia Shen, Christian Lesterlin, Jean-Yves Bouet and Yoshiharu Yamaichi.
Vertical and Horizontal Transmission of ESBL Plasmid from Escherichia coli O104:H4
Genes 2020, 11, 1207
2020 Oct doi:10.3390/genes11101207
Alix Meunier, François Cornet, Manuel Campos.
Bacterial cell proliferation: from molecules to cells
FEMS Microbiology Reviews
2020 Sep
Guilhas, B., Walter, J.-C., Rech, J., David, G. Walliser, N.-O. Palmeri, J., Mathieu-Demazière, C., Parmeggiani, A., Bouet, J.-Y., Le Gall, A. and Nollmann, M.
ATP-driven separation of liquid phase condensates in bacteria
Molecular Cell. 79:293-303
2020 Aug
Audibert S.*, Tanguy-le-Gac N.*, Rech J., Bouet J.Y., Bystricky K. and Lane D. .
Role of centromere sites in activation of ParB proteins for partition complex assembly
PLoS ONE 15(5):e0226472
2020 May 10.1371/journal.pone.0226472, 2020
Guynet, C., Nicolas, E. Ton-Hoang, B., Bouet, J.-Y. and Hallet, B.
First biochemical steps on bacterial transposition pathways
Methods in Molecular Biology. Chapter book Horizontal gene transfer. 2075:157-177
2020 Feb
C. Planchenault, M. Pons, C. Schiavon, P.Siguier, J. Rech, C. Guynet, J. Dauverd-Girault, J. Cury, E. Rocha, I. Junier, F. Cornet and O. Espeli.
Intracellular positioning systems limit the entropic eviction of secondary replicons toward the nucleoid edges in bacterial cells
J. Mol. Biol
2020 Feb 432(3): 745-61
C. Guynet, P.T.N. Le, M. Chandler, B. Ton-Hoang.
Detection and Characterization of Transposons in Bacteria
Horizontal Gene Transfer
2020 Feb Humana, New York, NY: 81-90
Jean-Yves Bouet and Barbara E. Funnell.
Plasmid Localization and Partition in Enterobacteriaceae
EcoSalPlus. 8(2)
2019 Jun doi:10.1128/ ecosalplus.ESP-0003-2019
Debaugny, R.E., Sanchez, A., Rech, J., Labourdette, D., Dorignac, J., Geniet, F., Palmeri, J., Parmeggiani, A., Boudscoq, F., Anton Leberre, V., Walter, J.-C. and Bouet, J.-Y.
A conserved mechanism drives partition complex assembly on bacterial chromosomes and plasmids
Molecular Systems Biology
2018 Nov 14: e8516
Campos M, Govers SK, Irnov I, Dobihal GS, Cornet F, Jacobs-Wagner C..
Genomewide phenotypic analysis of growth, cell morphogenesis, and cell cycle events in Escherichia coli.
Mol Syst Biol. 14(6):e7573. doi: 10.15252/msb.20177573.
2018 Jun PMID:29941428
Diaz R. E., Sanchez A., Anton Le Berre V. and Bouet J.-Y.
High Resolution Chromatin Immunoprecipitation: ChIP-sequencing
Methods in Molecular Biology. In book: The Bacterial Nucleoid, pp.61-73.
2017 Aug
Walter, J.-C., Dorignac, J., Lorman, V., Rech, J., Bouet, J.-Y., Nollmann, M., Palmeri, J., Parmeggiani, A. and Geniet, F.
Surfing on protein waves: proteophoresis as a mechanism for bacterial genome partitioning
Physical Revue Letter,119: 028101
2017 Jul Preprint on arXiv 1702.07372v1
Pillet, F., Passot, F., Pasta, F., Anton Leberre, V. and Bouet, J.-Y.
ParB-parS binding specificities in Burkholderiales reveal a ParABS sub-family allowing evolution and emergence of partitioning systems
Plos One
2017 May 12(5): e0177056
Le Gall, A., Cattoni, D., Guilhas, B., Mathieu-Demazière, C., Oudjedi, L., Fiche, J.-B., Rech, J., Murray, H., *Bouet, J.-Y. and *Nollmann, M.
Bacterial partition complexes segregate within the volume of the nucleoid
Nature Communications
2016 Jul 7:12107
Du WL, Dubarry N, Passot FM, Kamgoué A, Murray H, Lane D, Pasta F.
Orderly Replication and Segregation of the Four Replicons of Burkholderia cenocepacia J2315
PLoS Genet. 18;12(7):e1006172
2016 Jul
Sayyed HE, Le Chat L, Lebailly E, Vickridge E, Pages C, Cornet F, Cosentino Lagomarsino M, Espéli E..
Mapping Topoisomerase Iv Binding and Activity Sites on the E . Coli Genome
PLoS Genet
2016 Jan
Fournes F, Crozat E, Pages C, Tardin C, Salomé L, Cornet F*, Rousseau P*.
FtsK translocation inhibits recombination at a Xer site involved in horizontal gene transfer
Proc Nat Acad Sci USA
2016 Jan
Sanchez, A., Cattoni, D., Walter, J.-C., Rech, J., Parmeggiani, A., Nollmann, M. and Bouet, J.-Y.
Stochastic self-assembly of ParB proteins builds the bacterial DNA segregation apparatus
Cell Systems
2015 Jul 1:163-73
Diaz, R, Rech, J. and Bouet, J.-Y.
Imaging centromere-based incompatibilities: insights into the mechanism of incompatibility mediated by low-copy number plasmids
Plasmid
2015 Jul 80:54-62
Billard-Pomares, T., Fouteau, S., Jacquet, M.E., Roche, D., Barbe, V., Castellanos, M., Bouet, J.-Y., Cruveiller, S., Médigue, C., Blanco, J., Clermont, O., Denamur, E. and Branger, C.
Characterization of a P1-like bacteriophage encoding an SHV-2 extended-spectrum β-lactamase from an Escherichia coli strain
Antimicrob Agents CH
2014 Nov 58(11):6550-7
Bouet, J.-Y., Stouf, M., Lebailly, E. and Cornet, F.
Mechanisms for chromosome segregation
Current Opinion in Microbiology
2014 Oct 22:60-65
2014 Jan Crozat E, Fournes F, Cornet F, Hallet B, Rousseau P. (2014) Resolution of Multimeric Forms of Circular Plasmids and Chromosomes. In Plasmid Biology, M Tomalsky and JC Alonso Ed., ASM press. pp 251-273.microbiolspec 2(5): doi:10.1128/microbiolspec.PLAS-0025-2014.
2014 Jan Crozat E, Rousseau P, Fournès F, Cornet F. (2014) The FtsK Family of DNA Translocases Finds the Ends of Circles. J Mol Microbiol Biotechnol 2014;24:396–408 DOI: 10.1159/000369213.
2014 Jan Bouet J-Y, Souf M, Lebailly E, Cornet F. (2014) Mechanisms for chromosome segregation. Current Opinion in Microbiology 2014, 22:60–65.
2014 Jan Diagne CT, Salhi M, Crozat E, Salomé L, Cornet F, Rousseau P, Tardin C. (2014) TPM analyses reveal that FtsK contributes both to the assembly and the activation of the XerCD-dif recombination synapse. Nucleic Acids Res 42: 1721–1732.
Sanchez, A., Rech, J., Gasc, C. and Bouet, J.-Y.
Insight into centromere binding properties of ParB proteins: Identification of a secondary centromere binding motif essential for bacterial genome maintenance
Nucleic Acids Research
2013 Aug 41(5):3094-103
Ah-Seng, Y., Rech, J., Lane, D. and Bouet, J.-Y.
Defining the role of ATP hydrolysis in mitotic segregation of bacterial plasmids
PLOS Genetics
2013 Jul 9(12):1-13. doi:10.1371/journal.pgen.1003956
Pillet, F., Sanchez, A., Formosa, C., Séverac, M., Trévisiol, E., *Bouet, J.-Y. and *Anton-Leberre, V..
Dendrimer functionalization of gold surface improves the measurement of Protein-DNA interactions by Surface Plasmon Resonance imaging
Biosensors and Bioelectronics
2013 Jun 43:148-54
2013 Jan Stouf M, Meile J-C, Cornet F. (2013) FtsK actively segregates sister chromosomes in Escherichia coli. Proc Nat Acad Sci USA 110, 11157–11162.
2013 Jan Le Bourgeois P, Cornet F. (2013) Chromosome Dimer Resolution by Site-Specific Recombination. In: Stanley Maloy and Kelly Hughes, editors. Brenner’s Encyclopedia of Genetics 2nd edition, Vol 1. San Diego: Academic Press; 2013. p. 551–554.
Passot, F.M., Calderon, V., Fichant, G., Lane, D. and Pasta, F.
Centromere binding and evolution of chromosomal partition systems in the Burkholderiales
J. Bacteriol. 194(13):3426-36
2012 Jul
2012 Jan Plénat T, Tardin C, Rousseau P, Salomé L. (2012) High-throughput single-molecule analysis of DNA-protein interactions by tethered particle motion. Nucleic Acids Res. 40: e89.
2012 Jan Nolivos S, Touzain F, Pages C, Coddeville M, Rousseau P, El Karoui M, Le Bourgeois P, Cornet F. (2012) Co-evolution of segregation guide DNA motifs and the FtsK translocase in bacteria: identification of the atypical Lactococcus lactis KOPS motif. Nucl. Acid. Res. 40, 5535-5545.
Meile, J.C., Mercier, R., Stouf, M., Pages, C., Bouet*, J.-Y. and Cornet*, F.
The terminal region of the E. coli chromosome localises at the periphery of the nucleoid
BMC Microbiology
2011 Nov 11:28.
Pillet#, F., Sanchez#, A., Lane, D., Leberre, V. and Bouet, J.-Y.
Centromere binding specificity in assembly of the F plasmid partition complex
Nucleic Acids Research
2011 Aug 39(17): 7477–7486
2011 Jan Meile J-C, Mercier R, Pages C, Bouet J-Y, Cornet F. (2011) The terminal region of the E. coli chromosome localises at the periphery of the nucleoid. BMC Microbiol 11:28
2011 Jan Deghorain M, Pages C, Meile J-C, Stouf M, Capiaux H, Mercier R, Lesterlin C, Hallet B, Cornet F. (2011). A defined terminal region of the E. coli chromosome shows late segregation and high FtsK activity. PLoS One 6:e22164.
Dubarry, N., Du, W., Lane, D. and Pasta, F.
Improved electrotransformation and decreased antibiotic resistance of the cystic fibrosis pathogen Burkholderia cenocepacia strain J2315
Appl Environ Microbiol. 76(4):1095-102
2010 Jul
2010 Jan Nolivos S, Pages C, Rousseau P, Le Bourgeois P, Cornet F. (2010) Are two better than one? : Analysis of an FtsK/Xer recombination systems that uses a single recombinase. Nucl. Acid. Res. 38:6477-6489.
2010 Jan Manghi M, Tardin C, Baglio J, Rousseau P, Salomé L, Destainville N. (2010) Probing DNA conformational changes with high temporal resolution by tethered particle motion. Phys Biol. 7: doi: 10.1088/1478-3975/7/4/046003.
Ah-Seng, Y., Lopez, F., Pasta, F., Lane, D., and Bouet, J.-Y.
Dual role of DNA in regulating ATP hydrolysis by the SopA partition protein
J. Biol. Chem
2009 Aug 284(44): 30067-75
2009 Jan Sivanathan V, Emerson JE, Pages C, Cornet F, Sherratt DJ, Arciszewska L. (2009) KOPS-guided DNA translocation by FtsK safeguards Escherichia coli chromosome segregation. Mol Microbiol 71:1031-1042.
Bouet, J.-Y. and Lane, D.
Molecular basis of the supercoil deficit induced by the mini-F plasmid partition complex
J. Biol. Chem
2009 Jan 284(1): 165-73.
Castaing, J.-P., *Bouet, J.-Y. and Lane, D.
F plasmid partition depends on interaction of SopA with non-specific DNA
Mol. Microbiol
2008 Aug 74: 1000-1011
Ravin, N.V., Rech, J. and Lane, D.
Extended function of plasmid partition genes: the Sop system of linear phage-plasmid N15 facilitates late gene expression
J. Bact. 190: 3538-45
2008 Jul
2008 Jan Lesterlin C, Pages C, Dubarry N, Dasgupta S, Cornet F. (2008) Asymmetry of chromosome replichores renders the DNA translocase activity of FtsK essential for cell division and cell shape maintenance in E. coli. PloS Genetics, 4:e1000288.
Bouet J.-Y., Nördström, K. and Lane, D.
Plasmid partition and incompatibility - the focus shifts
Mol. Microbiol
2007 Aug 65: 1405-1414
2007 Jan Bigot S, Sivanathan V, Possoz C, Barre F-X, Cornet F. (2007) FtsK, a literate chromosome segregation machine. Mol Microbiol 64:1434-41.
Bouet J.-Y. , Ah-Seng, Y., Benmeradi, N. and Lane, D..
Polymerization of SopA partition ATPase: regulation by DNA binding and SopB
Mol. Microbiol
2007 Jan 63: 468-481
Bouet, J.-Y., Bouvier, M. and Lane, D.
Concerted action of plasmid maintenance functions: partition complexes create a requirement for dimer resolution
Mol. Microbiol
2006 Aug 62: 1447-1459
Dubarry, N., Pasta, F. and Lane, D.
ParABS systems of the four replicons of Burkholderia cenocepacia: new chromosome centromeres confer partition specificity
J. Bacteriol. 188: 1489-1496
2006 Jul
2006 Jan Bigot S, Saleh OA, Cornet F, Allemand J-F, Barre F-X. (2006) Oriented loading of FtsK on KOPS. Nat Struct Mol Biol 13:1026-1028.
2005 Jan Lesterlin C, Mercier R, Boccard F, Barre F-X, Cornet F. (2005) Roles for replichores and macrodomains in segregation of the E. coli chromosome. EMBO Reports 6:557-562.
2005 Jan Val M-E, Bouvier M, Campos J, Sherratt DJ, Cornet F, Mazel D, Barre F-X. (2005) The single stranded genome of phage CTX is the form used for integration into the genome of Vibrio cholerae. Mol Cell. 19:559-566.
2005 Jan Bigot S, Saleh OA, Lesterlin C, Pages C, El Karoui M, Dennis C, Grigoriev M, Allemand J-F, Barre F-X, Cornet F. (2005) KOPS: DNA motifs that control segregation of the E. coli chromosome by orienting the FtsK translocase. EMBO J 24:3770-3780.
Bouet, J.-Y., Rech, J., Egloff, S., Biek, D.P. and Lane, D.
Probing plasmid partition with centromere-based incompatibility
Mol. Microbiol
2005 Jan 55: 511-525.
Gordon, S., Rech, J., Lane, D. and Wright, A.
Kinetics of plasmid segregation in Escherichia coli
Mol. Microbiol. 51: 461-469
2004 Jul
Ravin, N.V., Rech, J. and Lane, D.
Mapping of functional domains in F plasmid partition proteins reveals a bipartite SopB-recognition domain in SopA
J. Mol. Biol. 329: 875-889
2003 Jul
Godfrin-Estevenon AM, Pasta F. and Lane D.
The parAB gene products of Pseudomonas putida exhibit partition activity in both P. putida and Escherichia coli
Mol. Microbiol. 43: 39-50
2002 Jul
Fung, E., Bouet, J.-Y. and Funnell, B.E.
Probing the ATP binding site of P1 ParA: partition and repression have different requirements for ATP binding and hydrolysis
EMBO J
2001 Aug 20: 4901-4911
#Lemonnier, M., #Bouet, J.-Y., Libante, V. and Lane, D.
Disruption of the F plasmid partition complex in vivo by partition protein SopA
Mol. Microbiol
2000 Aug 38: 493-503
Bouet, J.-Y., Surtees, J.A. and Funnell, B.E.
Stoichiometry of P1 plasmid partition complexes
J. Biol. Chem
2000 Mar 275: 8213-8219
Bouet, J-Y. and Funnell, B.E.
P1 ParA interacts with the P1 partition complex at parS and an ATP-ADP switch controls ParA activities
EMBO J
1999 Mar 18: 1415-1424
Bouet, J.-Y., Krisch, H.M. and Louarn, J.-M.
Ndd, the Bacteriophage T4 protein that disrupts the Escherichia coli nucleoid, has a DNA binding activity
J. Bacteriol. 180: 5227-5230
1998 Jun
Bouet, J.-Y.
Caractérisation du gène ndd du bactériophage T4. Etude de l'effet de la protéine Ndd sur le nucléoïde bactérien
Thèse de doctorat de l’Université Paul Sabatier (Toulouse III)
1996 Jun
Bouet, J.-Y., Campo, N.J., Krisch, H.M. and Louarn, J.-M.
The effects on Escherichia coli of the expression of the cloned bacteriophage T4 nucleoid disruption (ndd) gene
Mol. Microbiol. 20: 519-528
1996 Apr
Repoila, F., Tetard, F., Bouet, J.-Y. and Krisch, H.M.
Genomic polymorphism in the T-even bacteriophage
EMBO J. 13:4181-92
1994 Aug
Bouet, J.-Y., Woszczyk, J., Repoila, F., François, V., Louarn, J.-M. and Krisch, H.M. .
Direct PCR sequencing of the ndd gene of bacteriophage T4: Identification of a product involved in bacterial nucleoid disruption
Gene. 144: 9-16
1994 Apr

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