Team publications Polard
The RecA-directed recombination pathway of natural transformation initiates at chromosomal replication forks in the pneumococcus
Proceedings of the National Academy of Sciences (PNAS)
Assembly mechanism and cryoEM structure of RecA recombination nucleofilaments from Streptococcus pneumoniae
Nucleic Acids Research
Tight Interplay between Replication Stress and Competence Induction in Streptococcus pneumoniae
The alternative sigma factor σX mediates competence shut-off at the cell pole in Streptococcus pneumoniae
Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events
Direct Visualization of Horizontal Gene Transfer by Transformation in Live Pneumococcal Cells Using Microfluidics
Recombination of the Phase-Variable spnIII Locus Is Independent of All Known Pneumococcal Site-Specific Recombinases.
Journal of Bacteriology
Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome
Methods Mol Biol
Evidence that regulation of intramembrane proteolysis is mediated by substrate gating during sporulation in Bacillus subtilis.
Fine-tuning cellular levels of DprA ensures transformant fitness in the human pathogen Streptococcus pneumoniae.
Dynamic Modeling of Streptococcus pneumoniae Competence Provides Regulatory Mechanistic Insights Into Its Tight Temporal Regulation
2018 Jul 9:1637. doi:10.3389/fmicb.2018.01637.
Dynamic Modeling of Streptococcus pneumoniae Competence Provides Regulatory Mechanistic Insights Into Its Tight Temporal Regulation.
Frontiers in Microbiology
Nascent teichoic acids insertion into the cell wall directs the localization and activity of the major pneumococcal autolysin LytA
The Cell Surface
Real-time imaging of specific genomic loci in eukaryotic cells using the ANCHOR DNA labelling system
2018 May https://doi.org/10.1016/j.ymeth.2018.04.008
Peptidoglycan O-acetylation is functionally related to cell wall biosynthesis and cell division in Streptococcus pneumoniae.
A programmed cell division delay preserves genome integrity during natural genetic transformation in Streptococcus pneumoniae.
Bacterial RadA is a DnaB-type helicase interacting with RecA to promote bidirectional D-loop extension
Bacterial RadA is a DnaB-type helicase interacting with RecA to promote bidirectional D-loop extension.
Direct interaction between exocyst and Wave complexes promotes cell protrusions and motility.
Journal of Cell Science
Orderly Replication and Segregation of the Four Replicons of Burkholderia cenocepacia J2315.
Fine-tuning of choline metabolism is important for pneumococcal colonization.
Co-Inactivation of GlnR and CodY Regulators Impacts Pneumococcal Cell Wall Physiology.
RecFOR is not required for pneumococcal transformation but together with XerS for resolution of chromosome dimers frequently formed in the process.
Bacterial transformation: distribution, shared mechanisms and divergent control.
Nature Reviews Microbiology
Streptococcus pneumoniae, le transformiste.
Trends in Microbiology
Postreplication targeting of transformants by bacterial immune systems?
Trends in Microbiology
Natural genetic transformation generates a population of merodiploids in Streptococcus pneumoniae.
Midcell recruitment of the DNA uptake and virulence nuclease, EndA, for pneumococcal transformation.
The DpnI/DpnII pneumococcal system, defense against foreign attack without compromising genetic exchange.
Mobile Genetic Elements
Programmed protection of foreign DNA from restriction allows pathogenicity island exchange during pneumococcal transformation.
Direct involvement of DprA, the transformation-dedicated RecA loader, in the shut-off of pneumococcal competence.
ComE/ComE~P interplay dictates activation or extinction status of pneumococcal X-state (competence).
Structure-function analysis of pneumococcal DprA protein reveals that dimerization is crucial for loading RecA recombinase onto DNA during transformation.
Induction of competence for genetic transformation by antibiotics: convergent evolution of stress responses in distant bacterial species lacking SOS?
Current Opinion in Microbiology
Role of the single-stranded DNA-binding protein SsbB in pneumococcal transformation: maintenance of a reservoir for genetic plasticity.
The C-terminal domain of the bacterial SSB protein acts as a DNA maintenance hub at active chromosome replication forks.